Abstract
New compounds with chemotherapeutic activity are sought after, and plants are an important source of these compounds. Four diterpenes, 19-deoxyicetexone, 7,20-dihydroanastomosine, icetexone and 19-deoxyisoicetexone, were isolated from the hexane-washed chloroform extract of Salvia ballotiflora. The cytotoxic activity of the hexane-washed chloroform extract and its four diterpenes were tested using the MTT assay against three tumor cell lines: HeLa (cervical cancer), A549 (lung cancer) and MCF7 (breast cancer), and two murine cell line: J774A.1 (epithelial cancer) and CT26 (colon cancer), and their IC50 values were determined. 19-Deoxyisoicetexone had the greatest effect on HeLa cells with IC50 of 3.2 μg/ml (9.36 μM), whereas hexane-washed chloroform extract had the best cytotoxic effect on A549 cells with an IC50 of 2.29 μg/ml. These effects of 19-deoxyisoicetexone and hexane-washed chloroform extract were with similar activity compared to cisplatin (IC50 = 1.06 μg/ml in HeLa cells, and 4.6 μg/ml (15.21 μM) in A549 cells).
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NC-X, AJA-C, MAZ-S, and ES-M carried out the experimental studies. SP-G conceived the study, participated in its design and coordination, wrote the manuscript, and helped draft the manuscript. All authors read and approved the final manuscript.
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Campos-Xolalpa, N., Alonso-Castro, Á.J., Sánchez-Mendoza, E. et al. Cytotoxic activity of the chloroform extract and four diterpenes isolated from Salvia ballotiflora. Rev. Bras. Farmacogn. 27, 302–305 (2017). https://doi.org/10.1016/j.bjp.2017.01.007
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DOI: https://doi.org/10.1016/j.bjp.2017.01.007