Abstract
Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, is a plant from the southern coast of Brazilian that is used for the treatment of premenstrual syndrome, menstrual disorders and genito-urinary infections. The aim of the present study was to determine the quality control parameters for rhizomes collected during different periods by pharmacopoeial and non-pharmacopoeial methods, and to develop and validate a HPLC-DAD method for quantitative control of marker substances. The measured parameters were: granulometric analysis (d50 = 0.21–0.48 mm), loss on drying (11.1–12.4%), total ash (4.9–5.7%), dry residue by extraction with acetone:water (7:3, v/v) (30.6–39.5%), total polyphenol content (8.5–15.8%), and chromatographic fingerprint by HPLC and TLC. Besides, the acetone:water (7:3, v/v) extraction solvent in combination with a turbo-extractor, yielded the crude extract with a significant increase in tannins (F4,20 = 37.0, p< 0.001). The antioxidant potential of the crude acetone:water (7:3, v/v) extract, as well as the ethyl acetate and water fractions obtained after the partition process was evaluated by DPPH and the results were, respectively: IC50 6.87, 5.91, and 6.92 μg/ml. The validation parameters for the HPLC-DAD method showed adequate specificity, precision and accuracy. The gallo- and epigallocatechin contents were, respectively, 0.8–2.7% and 1.2–2.2%. These data contribute to analysis of the pharmacognostic quality control of the commonly used part from this species.
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AB (Ph.D. student) collected and dried the plant material samples A and C, prepared their voucher specimens, worked on laboratorial tests with both samples, conducted all HPLC and TLC analysis, and wrote and formatted the article. TMAU collected and dried sample B of the plant material, prepared its voucher specimen, worked in laboratorial tests with this sample, and assisted with writing. GG (undergraduate student) assisted in laboratory work with sample B. EVSLM assisted in the project design and reviewed the manuscript. JCPM was responsible for the project concept and supervision of the study, as well as the writing and review of the manuscript.
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Blainski, A., Antonelli-Ushirobira, T.M., Godoy, G. et al. Pharmacognostic evaluation, and development and validation of a HPLC-DAD technique for gallocatechin and epigallocatechin in rhizomes from Limonium brasiliense. Rev. Bras. Farmacogn. 27, 162–169 (2017). https://doi.org/10.1016/j.bjp.2016.08.009
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DOI: https://doi.org/10.1016/j.bjp.2016.08.009