Abstract
Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, is used as a food additive because its leaves are a source of steviol glycosides. There are examples of tissue culture based on micropropagation and phytochemical production of S. rebaudiana leaves but there are few studies on adventitious root culture of S. rebaudiana. More than 90% of the plants used in industry are harvested indiscriminately. In order to overcome this situation, the development of methodologies that employ biotechnology, such as root culture, provides suitable alternatives for the sustainable use of plants. The aim of this study was to compare morpho-anatomical transverse sections of S. rebaudiana roots grown in vitro and in vivo. The in vitro system used to maintain root cultures consisted of a gyratory shaker under dark and light conditions and a roller bottle system. Transverse sections of S. rebaudiana roots grown in vitro were structurally and morphologically different when compared to the control plant; roots artificially maintained in culture media can have their development affected by the degree of media aeration, sugar concentration, and light. GC-MS and TLC confirmed that S. rebaudiana roots grown in vitro have the ability to produce metabolites, which can be similar to those produced by wild plants.
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TFOS and RVR (MSc students) carried out the laboratory work (preparing herbaria samples, chemical extraction, obtaining chromatograms, and plant anatomy studies). TPCC contributed to carrying out the root culture laboratory work and analysis. ALMA and LAS contributed in plant anatomy studies. JEG contributed to the chromatographic analysis. AJBO and RACG designed the study, supervised the laboratory work, and wrote the manuscript. All the authors have read the final manuscript and approved the submission.
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Reis, R.V., Chierrito, T.P.C., Silva, T.F.O. et al. Morpho-anatomical study of Stevia rebaudiana roots grown in vitro and in vivo. Rev. Bras. Farmacogn. 27, 34–39 (2017). https://doi.org/10.1016/j.bjp.2016.08.007
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DOI: https://doi.org/10.1016/j.bjp.2016.08.007