Abstract
The leaves of Garcinia gracilis Pierre, Clusiaceae, have been used as flavouring materials in food, with no previous reports of their biological activities and chemical constituents. In this study, the methanolic extract of G. gracilis afforded three compounds namely apigenin-8-C-α-L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranoside (1), 5-hydroxymethyl-2-furaldehyde, and vanillic acid. All of the isolates were initially evaluated for superoxide anion radical scavenging activity and a-glucosidase inhibitory effects. Compound 1, which was the major component, showed the most potent activities among these three isolates. Further biological evaluations revealed that compound 1 could prevent the pBR322 plasmid DNA damage induced by the photochemical reaction of riboflavin and protect P19-derived neurons from the oxidative stress condition induced by serum deprivation. It was concluded that the potent biological activities of G. gracilis could be attributed to the synergistic effect of compound 1 with other constituents found in the plant.
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Acknowledgements
This study was supported by the 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship of Chulalongkorn University. The authors would also like to thank The Research Instrument Center of the Faculty of Pharmaceutical Sciences at Chulalongkorn University for providing research facilities.
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CS (PhD student) contributed to the isolation and purification of the compounds, the running of the laboratory work, the analysis of the data, and the drafting of the paper. WN contributed to isolation and purification of the compounds. ST contributed to the cell-based assay of neuroprotective activity. PT and KL contributed to the critical reading of the manuscript. BS contributed to the plant collection, the supervision of the laboratory work and critical reading of the manuscript. All authors have read the final manuscript and approved the submission.
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Supasuteekul, C., Nonthitipong, W., Tadtong, S. et al. Antioxidant, DNA damage protective, neuroprotective, and α-glucosidase inhibitory activities of a flavonoid glycoside from leaves of Garcinia gracilis. Rev. Bras. Farmacogn. 26, 312–320 (2016). https://doi.org/10.1016/j.bjp.2016.01.007
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DOI: https://doi.org/10.1016/j.bjp.2016.01.007