Abstract
Alzheimer’s disease affects nearly 36.5 million people worldwide, and acetylcholinesterase inhibition is currently considered the main therapeutic strategy against it. Seaweed biodiversity in Brazil represents one of the most important sources of biologically active compounds for applications in phytotherapy. Accordingly, this study aimed to carry out a quantitative and qualitative assessment of Hypnea musciformis (Wulfen) J.V. Lamouroux, Ochtodes secundiramea (Montagne) MA. Howe, and Pterocladiella capillacea (S.G. Gmelin) Santelices & Hommersand (Rhodophyta) in order to determine the AChE effects from their extracts. As a matter of fact, the O. secundiramea extract showed 48% acetylcholinesterase inhibition at 400 (ig/ml. The chemical composition of the bioactive fraction was determined by gas chromatography-mass spectrometry (GC-MS); this fraction is solely composed of halogenated monoterpenes, therefore allowing assignment of acetylcholinesterase inhibition activity to them.
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Acknowledgments
The authors thank financial supports from CNPq and FAPESP, scholarship from CAPES to the first author, and grants from CNPq to MCMY, PC, and NSY. This study is part of thesis presented by the first author to the Graduate Programme in Plant Biodiversity and Environment, Institute of Botany, São Paulo, Brazil. The authors would also like to thank Prof. O. Vieira for proof-reading and editing the manuscript in English.
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LPM collected and identified seaweed samples, as well ascarried out lab experiments, analyzed the data, and wrote the manuscript. LRC performed chemical and chromatographic analyses and provided critical reading of the manuscript. MCMY and EMCL performed laboratory experiments involving biological studies. DCC and LZV carried out chemical studies. PC and NSY designed the study, supervised the laboratory work and contributed to critical reading of the manuscript. All authors have read the final manuscript and approved the submission.
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Machado, L.P., Carvalho, L.R., Young, M.C.M. et al. Evaluation of acetylcholinesterase inhibitory activity of Brazilian red macroalgae organic extracts. Rev. Bras. Farmacogn. 25, 657–662 (2015). https://doi.org/10.1016/j.bjp.2015.09.003
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DOI: https://doi.org/10.1016/j.bjp.2015.09.003