Abstract
The species Marcetia taxifolia (A. St.-Hil.) DC., Melastomataceae, which is endemic of the rupestrian fields of northeastern Brazil, contains a significant amount of flavonoids. In this work, the potential of the ethanolic extract of M. taxifolia as the active principle in a sunscreen photoprotection (UV-A and UV-B) formulation was investigated. The Liquid Chromatography High Performance-Diode Array Detector quantification (quercetin), total flavonoid content, antioxidant activity through 2.2-diphenyl-1- picrylhydrazil method, photoprotective activity against UV-B and UV-A radiation in vitro (spectrophotometric method) and potential for eye irritation using the methodology of the hen egg test-chorioallantoic membrane were performed in the extract. After that, the formulations were prepared using different concentrations of active ethanolic extract (5, 10, 20 and 30%) and the evaluation of the sun protection factor was carried out using the same methodology used for the crude extract. The crude extract showed UV-A photoprotection and low eye irritation in the hen egg test-chorioallantoic membrane test. All formulations containing M. taxifolia extract had ≥6 sun protection factor. Its shows the possibility to use this extracts as a sunscreen in pharmaceutical preparations.
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SCCC helped with the measurement of the in vitro antioxidant activity, total flavonoid and HPLC analysis. She also participated of the preparation of the formulations, interpretation of data and statistical analysis, evaluation of the in vitro photoprotective activity of sunscreens and the evaluation stability tests. CBD was in charge of the evaluation of the in vitro photoprotective activity of sunscreens. MBB helped with the measurement of the in vitro Eye Irritation Tests (HET-CAM) and carried out the interpretation of data and statistical analysis. CRCB conducted studies of the HPLC and the development of the sunscreen formulation. AB participated in the coordination of the project. All authors have read and approved the final manuscript.
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Costa, S.C.C., Detoni, C.B., Branco, C.R.C. et al. In vitro photoprotective effects of Marcetia taxifolia ethanolic extract and its potential for sunscreen formulations. Rev. Bras. Farmacogn. 25, 413–418 (2015). https://doi.org/10.1016/j.bjp.2015.07.013
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DOI: https://doi.org/10.1016/j.bjp.2015.07.013