Abstract
Solidago chilensis Meyen, Asteraceae, is traditionally used to treat inflammation. However, phytochemical and pharmacology investigations are lacking. This study evaluated the hypoglycemic and hypolipidemic effects of hydroalcoholic extract from S. chilensis aerial parts in rats. In oral glucose tolerance tests the rats received saline (0.5 ml/100 g) in control group (C), hydroalcoholic extract (125, 250 or 500 mg/kg p.o.; n = 6) or glibenclamide (10 mg/kg p.o.; n = 6). After 30 min, glucose (4 g/kg) was administered. Rats treated with hydroalcoholic extract 500 demonstrated decreased glucose levels at 180 min (−22.1%), when compared with group C, similar to glibenclamide. Moreover, treatment with hydroalcoholic extract 500 significantly increased the glycogen content in the liver and soleus muscle, and hydroalcoholic extract 250 specifically inhibited the enzyme maltase when compared with group C. Furthermore, all hyperglycemic rats treated with hydroalcoholic extract (125, 250 and 500) exhibited an accentuated decrease in total cholesterol levels (−36.8%, −36.7% and −41.3%, respectively). Our results suggest that hypoglycemic and hypolipidemic effects of hydroalcoholic extract could be associated with increased production and release of insulin as well as with insulinotropic and antioxidant effects.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alam, M.A., Subham, N., Chowdhury, S.A., Awal, M.A., Mostofa, M., Rashid, M.A., Hasan, C.M., Nahar, L., Sarker, S.D., 2011. Anthocephalus cadamba (Roxb.) Miq., Rubiaceae, extract shows hypoglycemic effect and eases oxidative stress in alloxan-induced diabetic rats. Rev. Bras. Farmacogn. 21, 155–164.
Alonso, J.R., 1998. Tratado de Fitomedicina: Bases Clínicas e Farmacológicas. Buenos Aires, ISIS Ediciones.
Apáti, P., Houghton, P.J., Kite, G., Steventon, G.B., Kery, A.J., 2006. In-vitro effect of flavonoids from Solidago canadensis extract on glutathione S-transferase. J. Pharm. Pharmacol. 58, 251–256.
Babu, P.V.A., Liu, D., Gilbert, E.R., 2013. Recent advances in understanding the antidiabetic actions of dietary flavonoids. J. Nutr. Biochem. 24, 1–28.
Bei, W.J., Guo, J., Wu, H.Y., Cao, Y., 2012. Lipid-regulating effect of traditional Chinese medicine: mechanisms of actions. Evid. Based Complement. Alternat. Med. 1, 1–19.
Brand-Williams, W., Cuvelier, M.E., Berset, C., 1995. Use of a free radical method to evaluate antioxidant activity. Lebenson. Wiss. Technol. 28, 25–30.
Bucciarelli, A., Minetti, A., Milczakovskig, C., Skliar, M., 2010. Evaluation of gastroprotective activity and acute toxicity of Solidago chilensis Meyen (Asteraceae). Pharmaceut. Biol. 48, 1025–1030.
Concea, 2012. Guia Brasileiro de Boas Práticas em Eutanásia em Animais: Conceitos e Procedimentos Recomendados. Brasília, 62 p.
Dahlqvist, A., 1984. Assay of intestinal disaccharidases. Scand. J. Clin. Lab. Invest. 44, 169–172.
Dai, F.J., Hsu, W.H., Huang, J.J., Wu, S.C., 2013. Effect of pigeon pea (Cajamus cajan L.) on high-fat diet-induced hypercholesterolemia in hamsters. Food Chem. Toxicol. 53, 384–393.
Diehl, K.H., Hull, R., Morton, D., Pfister, R., Rabemampianina, Y., Smith, D., Vidal, J.M., Vorstenbosch, C.V., 2001. A good practice guide to the administration of substances and removal of blood, including routes and volumes. J. Appl. Toxicol. 21, 15–23.
Facury-Neto, M.A., Fagundes, D.J., Beletti, M.E., Novo, N.F., Penha, S.Y.J.N., 2004. Systemic use of Solidago microglossa DC in the cicatrization of open cutaneous wounds in rats. Braz. J. Morphol. Sci. 21, 207–210.
Ferrer, J.C., Favre, C., Gomis, R.R., Fernández-Novell, J.M., Garcia-Rocha, M., Dela-Iglesia, N., Cid, E., Guinovart, J.J., 2003. Control of glycogen deposition. FEBS Lett. 546, 127–132.
Grover, J.K., Yadav, S., Vats, V., 2002. Medicinal plants of India with antidiabetic potential. J. Ethnopharmacol. 81, 81–100.
Hirany, S., O’Byrne, D., Devaraj, S., Jialal, I., 2000. Remnant-like particle-cholesterol concentrations in patients with type 2 diabetes mellitus and end-stage renal disease. Clin. Chem. 46, 667–672.
Jensen, J., Rustad, P.I., Kolnes, A.J., Lai, Y.C., 2011. The role of skeletal muscle glycogen breakdown for regulation of insulin sensitivity by exercise. Front. Physiol. 2, 1–11.
Jorge, A.P., Horst, H., Sousa, E., Pizzolatti, M.G., Silva, F.R., 2004. Insulinomimetic effects of kaempferitrin on glycaemia and on 14C-glucose uptake in rat soleus muscle. Chem. Biol. Interact. 149, 89–96.
Kappel, V.D., Frederico, M.J.S., Postal, B.G., Mendes, C.P., Cazarolli, L.H., Silva, F.R.M.B., 2013. The role of calcium in intracellular pathways of rutin in rat pancreatic islets: potential insulin secretagogue effect. Eur. J. Pharmacol. 702, 264–268.
Krisman, C.R., 1962. A method for the colorimetric estimation of glycogen with iodine. Anal. Biochem. 4, 14–23.
Li, W., Zhang, M., Gu, J., Meng, Z., Zhao, L.C., Zheng, Y., Chen, L., Yang, G.L., 2012. Hypoglycemic effect of protopanaxadiol-type ginsenosides and compound K on type 2 diabetes mice induced by high-fat diet combining with streptozotocin via suppression of hepatic gluconeogenesis. Fitoterapia 83, 192–198.
Liu, W., Zheng, Y., Zhang, Z., Yao, W., Gao, X., 2014. Hypoglycemic, hypolipidemic and antioxidant effects of Sarcandra glabra polysaccharide in type 2 diabetic mice. Food Funct. 22, 2850–2860.
Lorenzi, H., Matos, F.J.A., 2002. Plantas medicinais do Brasil: Nativas e exóticas. Instituto Plantarum de Estudos da Flora, São Paulo.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.
Mai, T.T., Chuyen, N.V., 2007. Anti-hyperglycemic activity of aqueous extract from flower buds of Cleistocalyx operculatus (Roxb.) Merr and Perry. Biosci. Biotechnol. Biochem. 71, 69–76.
Melo, A.M., Bittencourt, P., Nakutis, F.S., Silva, A.P., Cursino, J., Santos, G.A., Ashino, N.G., Velloso, L.A., Torsoni, A.S., Torsoni, M.A., 2011. Solidago chilensis Meyen hydroalcoholic extract reduces JNK/I (B pathway activation and ameliorates insulin resistance in diet-induced obesity mice. Exp. Biol. Med. (Maywood) 236, 1147–1155.
Meng, S., Cao, J., Feng, Q., Peng, J., Hu, Y., 2013. Roles of chlorogenic acid on regulating glucose and lipids metabolism: a review. Evid. Based Complement. Alternat. Med. 1, 1–11.
Modak, M., Dixit, P., Londhe, J., Ghaskadbi, S., Paul, A., 2007. Indian herbs and herbal drugs for the treatment of diabetes. J. Clin. Biochem. Nutr. 40, 163–173.
Negri, G., 2005. Diabetes melito: plantas e princípios ativos naturais hipoglicemiantes. Rev. Bras. Cienc. Farm 41, 121–142.
Park, J.B., Velasquez, M.T., 2012. Potential effects of lignan-enriched flaxseed powder on bodyweight, visceral fat, lipid profile and blood pressure in rats. Fitoterapia 83, 941–946.
Patel, D.K., Kumar, R., Laloo, D., Hemalatha, S., 2012. Diabetes mellitus: an overview on its pharmacological aspects and reported medicinal plants having antidiabetic activity. Asian Pac. J. Trop. Biomed. 2, 411–420.
Patil, R.N., Patil, R.Y., Ahirwar, A., Ahirwar, D., 2011. Evaluation of antidiabetic and related actions of some Indian medicinal plants in diabetic rats. Asian Pac. J. Trop. Dis. 4, 20–23.
Pereira, D.F., Cazarolli, L.H., Lavado, C., Mengatto, V., Figueiredo, M.S.R.B., Guedes, A., Pizzolatti, M.G., Silva, F.R.M.B., 2011. Effects of flavonoids on alpha-glucosidase activity: potential targets for glucose homeostasis. Nutrition 27, 1161–1167.
Pereira, D.F., Kappel, V.D., Cazarolli, L.H., Boligon, A.A., Athayde, M.L., Guesser, S.M., Da Silva, E.L., Silva, F.R.M.B., 2012. Influence of the traditional Brazilian drink Ilex paraguariensis tea on glucose homeostasis. Phytomecine 19, 868–877.
Piaulino, C.A., Carvalho, F.C., Almeida, B.C., Chaves, M.H., Almeida, F.R., Brito, S.M., 2013. The stem bark extracts of Cenostigma macrophyllum attenuates tactile allodynia in streptozotocin-induced diabetic rats. Pharm. Biol., 1243–1248.
Prabhakar, P.K., Prasadb, R., Ali, S., Doble, M., 2013. Synergistic interaction of ferulic acid with commercial hypoglycemic drugs in streptozotocin induced diabetic rats. Phytomedicine 20, 488–494.
Prasath, G.S., Subramanian, S.P., 2011. Modulatory effects of fisetin, a bioflavonoid, on hyperglycemia by attenuating the key enzymes of carbohydrate metabolism in hepatic and renal tissues in streptozotocin-induced diabetic rats. Eur. J. Pharmacol. 668, 492–496.
Raida, K., Nizar, A., Barakat, S., 2008. The effect de Crataegus aronica aqueous extract in rabbits fed with high cholesterol diet. Eur. J. Sci. Res. 22, 352–360.
Roman-Junior, W.A., Piato, A.L., Conterato, G.M.M., Wildner, S.M., Marcon, M., Mocelin, R., Emanuelli, M.P., Emanuelli, T., Nepel, A., Barison, A., Santos, C.A.M., 2015. Hypolipidemic effects of Solidago chilensis hydroalcoholic extract and its major isolated constituent quercetrin in cholesterol-fed rats. Pharm. Biol., https://doi.org/10.3109/13880209.2014.989622.
Silva, A.G., De-Sousa, C.P.G., Koehler, J., Fontana, J., Christo, A.G., Guedes-Bruni, R.R., 2010. Evaluation of an extract of Brazilian arnica (Solidago chilensis Meyen, Asteraceae) in treating lumbago. Phytother. Res. 24, 283–287.
Soares-Valverde, S.S., Azevedo, S.R.C., Tomassini, T.C.B., 2009. Utilização de CLAE, como paradigma na obtenção e controle do diterpenosolidagenona a partir de inflorescências de Solidago chilensis Meyen (arnica brasileira). Rev. Bras. Farm. 9, 196–199.
Tamura, E.K., Jimenes, J.K., Waismam, K., Gobbo, N.L., Peporine, L.N., Malpezzi, M.E.A.L., Marinho, E.A.V., Farsky, F.H.P., 2009. Inhibitory effects of Solidago chilensis Meyen hydroalcoholic extract on acute inflammation. J. Ethnopharmacol. 122, 478–485.
Teixeira, C.C., Rava, C.A., Da-Silva, P.M., Melchior, R., Argenta, R., Anselmi, F., Almeida, C.R., Fuchs, F.D., 2000. Absence of antihyperglycemic effect of jambolan in experimental and clinical models. J. Ethnopharmacol. 71, 343–347.
Torres, L.M.B., Akisue, M.K., Roque, N.F., 1987. Quercetrina em Solidago microglossa DC, a arnica do Brasil. Rev. Farm. Bioquím. Univ. S. Paulo 23, 33–40.
Trovato, A., Monforte, M.T., Barbera, R., Rossito, A., Galati, E.M., Forestieri, A.M., 1996. Effects of fruit juices of Citrus sinensis L. and Citrus limon L. on experimental hypercholesterolemia in the rat. Phytomedicine 2, 221–227.
Warjeet, S.L., 2011. Traditional medicinal plants of Manipur as antidiabetics. J. Med. Plants Res. 5, 677–687.
Xie, W., Wang, W., Su, H., Xing, D., Cai, G., Du, L., 2007. Hypolipidemic mechanisms of Ananas comosus L. leaves in mice: different from fibrates but similar of statins. J. Pharmacol. Sci. 103, 267–274.
Zhao, J., Zhang, W., Zhu, X., Zhao, D., Wang, K., Wang, R., Qu, W., 2011. The aqueous extract of Asparagus officinalis L. by-product exerts hypoglycaemic activity in streptozotocin-induced diabetic rats. J. Sci. Food Agric. 91, 2095–2099.
Zimmet, P., 2000. Globalization, coca-colonization and the chronic disease epidemic: can the Doomsday scenario be averted. J. Intern. Med. 247, 301–310.
Acknowledgements
This work was supported by the Unochapecó [modality Art. 171 — FUMDES], CNPq-PIBIC (edital N° 228/Reitoria/2014), PIBIC-FAPE (edital N° 121/Reitoria/2013) and FAPESC.
Author information
Authors and Affiliations
Contributions
MS, APS and MG contributed in all steps of this study. EI, FB, GDS, AS, RC and RM contributed to biological studies. EF, GP and SMW contributed to biochemical analyses. LZ and WARJ have guided the laboratory work and contributed to design of the study. All the authors have read the final manuscript and approved the submission.
Corresponding author
Ethics declarations
The authors declare no conflicts of interest.
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Schneider, M., Sachett, A., Schönell, A.P. et al. Hypoglycemic and hypolipidemic effects of Solidago chilensis in rats. Rev. Bras. Farmacogn. 25, 258–263 (2015). https://doi.org/10.1016/j.bjp.2015.05.001
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1016/j.bjp.2015.05.001