Skip to main content
SpringerLink
Log in
Menu
Find a journal Publish with us
Search
Cart
  1. Home
  2. Revista Brasileira de Farmacognosia
  3. Article

HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities

  • Original Article
  • Open Access
  • Published: 23 March 2015
  • volume 25, pages 134–141 (2015)
Download PDF

You have full access to this open access article

Revista Brasileira de Farmacognosia Aims and scope Submit manuscript
HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities
Download PDF
  • Rana M. Ibrahim1,
  • Ali M. El-Halawany1,2,
  • Dalia O. Saleh3,
  • El Moataz Bellah El Naggar4,
  • Abd El-Rahman O. El-Shabrawy1 &
  • …
  • Seham S. El-Hawary1 
  • 229 Accesses

  • 122 Citations

  • Explore all metrics

  • Cite this article

Abstract

Securigera securidaca (L.) Degen & Döefl., Fabaceae, has been widely used in the Iranian, Indian and Egyptian folk medicine as antidiabetic and anti-hyperlipidemic remedy. Phenolic profiling of the ethanolic extract (90%) of the flowers of S. securidaca was performed via HPLC-DAD-MS/MS analysis in the positive and negative ion modes. The total polyphenols and flavonoids in the flowers were determined colorimetrically, and the quantification of their components was carried out using HPLC-UV. Total phenolics and flavonoids estimated as gallic acid and rutin equivalents were 82.39 ± 2.79 mg/g and 48.82 ± 1.95 mg/g of the dried powdered flowers, respectively. HPLC-DAD-MS/MS analysis of the extract allowed the identification of 39 flavonoids and eight phenolic acids. Quantitative analysis of some flavonoids and phenolics (mg/100 g powdered flowers) revealed the presence of isoquercetrin (3340 ± 2.1), hesperidin (32.09 ± 2.28), naringin (197.3 ± 30.16), luteolin (10.247 ± 0.594), chlorogenic acid (84.22 ± 2.08), catechin (3.94 ± 0.57) and protocatechuic acid (34.4 ± 0.15), in the extract. Moreover, the acute toxicity, hypoglycemic and hypolipidemic effects of the extract were investigated using alloxan induced diabetes in rats in a dose of 100, 200, and 400 mg/kg bwt. The ethanolic extract was safe up to a dose of 2000 mg/kg. All tested doses of the flower extract showed marked decrease in blood glucose level by 31.78%, 66.41% and 63.8% at 100, 200 and 400 mg/kg bwt, respectively, at p < 0.05. Regarding the anti-hyperlipidemic effect, a dose of 400 mg/kg of the flower extract showed the highest reduction in serum triacylglycerides and total cholesterol levels (68.46% and 51.50%, respectively at p < 0.05). The current study proved the folk use of the flowers of S. securidaca as anti-diabetic and anti-hyperlipidemic agent which could be attributed to its high phenolic content.

Download to read the full article text

Use our pre-submission checklist

Avoid common mistakes on your manuscript.

References

  • Ali, A.A., Mohamed, M.H., Kamel, M.S., Fouad, M.A., Spring, O., 1998. Studies on Securigera securidacea (L.) Deg. et Dorfl. (Fabaceae) seeds an antidiabetic Egyptian folk medicine. Pharmazie 53, 710–715.

    CAS  PubMed  Google Scholar 

  • Allain, C., Poon, L., Chan, C., Richmond, W., Paul, C., 1974. Enzymatic determination of total serum cholesterol. Clin. Chem. 20, 470–475.

    Article  CAS  PubMed  Google Scholar 

  • Azarmiy, Y., Zakheri, A., Alaf, A., Fathiazad, F., Fakhrjou, A., Andalib, S., Maaleki, D., Babaei, H., Garjani, A., 2009. The effect of total extract of Securigera securidaca L. seed on thoracic aorta function in high-fat fed rats. Pharm. Sci. 15, 83–92.

    Google Scholar 

  • Bahadoran, Z., Mirmiran, P., Azizi, F., 2013. Dietary polyphenols as potential nutraceuticals in management of diabetes: a review. J. Diabetes Metab. Disord. 12, 43.

    Article  PubMed  PubMed Central  Google Scholar 

  • Brahmachari, G., 2011. Bio-flavonoids with promising antidiabetic potentials: a critical survey. In: Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. Res Signpost, Trivandrum, Kerala, India, pp. 187–221.

    Google Scholar 

  • del Pilar Nicasio-Torres, M., Meckes-Fischer, M., Aguilar-Santamaría, L., Garduño-Ramírez, M., Chávez-Ávila, V., Cruz-Sosa, F., 2012. Production of chlorogenic acid and isoorientin hypoglycemic compounds in Cecropia obtusifolia calli and in cell suspension cultures with nitrate deficiency. Acta Physiol. Plant. 34, 307–316.

    Article  Google Scholar 

  • Fathi, A.F., Alaf, A.N., Zakheri, A., Andalib, S., Maleki Dizaji, N., Gharah Baghian, A., Ghorbani, H.A., Fakhrjou, A., Garjani, A., 2010. Hypolipidemic and antioxidant effects of Securigera securidaca L. seed in high fat fed rats. Pharm. Sci. 15, 293–301.

    Google Scholar 

  • Figueirinha, A., Paranhos, A., Pérez-Alonso, J., Santos-Buelga, C., Batista, M., 2008. Cymbopogon citratus leaves characterization of flavonoids by HPLCPDA-ESI/MS/MS and an approach to their potential as a source of bioactive polyphenols. Food Chem. 110, 718–728.

    Article  CAS  Google Scholar 

  • Folador, P., Cazarolli, L., Gazola, A., Reginatto, F., Schenkel, E., Silva, F., 2010. Potential insulin secretagogue effects of isovitexin and swertisin isolated from Wilbrandia ebracteata roots in non-diabetic rats. Fitoterapia 81, 1180–1187.

    Article  CAS  PubMed  Google Scholar 

  • Fossati, P., Prencipe, L., 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem. 28, 2077–2080.

    Article  CAS  PubMed  Google Scholar 

  • Goupy, P., Hugues, M., Boivin, P., Amiot, M., 1999. Antioxidant composition and activity of barley (Hordeum vulgare) and malt extracts and of isolated phenolic compounds. J. Sci. Food Agric. 79, 1625–1634.

    Article  CAS  Google Scholar 

  • Hertog, M., Hollman, P., Katan, M., 1992. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J. Agric. Food Chem. 40, 2379–2383.

    Article  CAS  Google Scholar 

  • Hosseinzadeh, H., Ramezani, M., Danaei, A.R., 2002. Antihyperglycaemic effect and acute toxicity of Securigera Securidaca L. seed extracts in mice. Phytother. Res. 16, 745–747.

    Article  CAS  PubMed  Google Scholar 

  • IDF, 2011. Diabetes Atlas. 5th ed. International Diabetes Federation, Brussels, Belgium.

    Google Scholar 

  • Islam, M.N., Ishita, I.J., Jung, H.A., Choi, J.S., 2014. Vicenin 2 isolated from Artemisia capillaris exhibited potent anti-glycation properties. Food Chem. Toxicol. 69, 55–62.

    Article  CAS  PubMed  Google Scholar 

  • Jadhav, R., Puchchakayala, G., 2012. Hypoglycemic and antidiabetic activity of flavonoids: boswellic acid, ellagic acid, quercetin, rutin on streptozotocinnicotinamide induced type 2 diabetic rats. Inter. J. Pharmacy Pharm. Sci. 4, 251–256.

    CAS  Google Scholar 

  • Jung, U.J., Lee, M.K., Jeong, K.S., Choi, M.S., 2004. The hypoglycemic effects of hesperidin and naringin are partly mediated by hepatic glucose-regulating enzymes in C57BL/KsJ-db/db mice. J. Nutr. 134, 2499–2503.

    Article  CAS  PubMed  Google Scholar 

  • Kim, J., Ju, B., Choi, W., Kim, S., 2006. Hypoglycemic and anti-hyperlipidemic effect of four Korean medicinal plants in alloxan induced diabetic rats. Am. J. Biochem. Biotechnol. 2, 154–160.

    Article  Google Scholar 

  • Krishnakumar, K., Augusti, K., Vijayammal, P., 2000. Anti-peroxidative and hypoglycaemic activity of Salacia oblonga extract in diabetic rats. Pharm. Biol. 38, 101–105.

    Article  CAS  PubMed  Google Scholar 

  • Kumar, M., Verma, D., 2011. Anti-diabetic and anti-hyperlipidemic effect of Morinda citrofolia and Coccinia indica in alloxan induced diabetic rats. Pharmacol. Online 2, 307–311.

    CAS  Google Scholar 

  • Latha, R., Daisy, P., 2011. Insulin-secretagogue antihyperlipidemic and other protective effects of gallic acid isolated from Terminalia bellerica Roxb. in streptozotocin-induced diabetic rats. Chem. Biol. Interact. 189, 112–118.

    Article  CAS  PubMed  Google Scholar 

  • Lorke, D., 1983. A new approach to practical acute toxicity testing. Arch. Toxicol. 54, 275–287.

    Article  CAS  PubMed  Google Scholar 

  • Mahdi, P., Mohammad, E., Ali-Akbar, M., Karim, P., 2011. To study the effects of Securigera securidaca (L.) seed against alloxan-induced hyperglycemia. J. Med. Plants Res. 5, 3188–3191.

    Google Scholar 

  • Mattila, P., Astola, J., Kumpulainen, J., 2000. Determination of flavonoids in plant material by HPLC with diode-array and electro-array detections. J. Agric. Food Chem. 48, 5834–5841.

    Article  CAS  PubMed  Google Scholar 

  • Meenakshi, S., Manicka, G., Tamil, M., Arumugam, M., Balasubramanian, T., 2009. Total flavanoid and in vitro antioxidant activity of two seaweeds of Rameshwaram coast. Glob. J. Pharmacol. 3, 59–62.

    Google Scholar 

  • Neshwari, D., Biren, S., Singh, S., Singh, C., Lukesh, D., Amitabh, D., 2012. Antihyperglycemic effect of aqueous and ethanol extract of aerial part of Osbeckia nepalensis hook in alloxan induced diabetic rats. Int. J. PharmTech Res. 4, 233–244.

    Google Scholar 

  • Osweiler, G., Carson, T., Buck, W., Van Gelder, G., 1985. Clinical and Diagnostic Veterinary Toxicology. Kendall/Hunt Publishing Company.

    Google Scholar 

  • Pitchai, D., Manikkam, R., 2012. Hypoglycemic and insulin mimetic impact of catechin isolated from Cassia fistula: a substantiate in silico approach through docking analysis. Med. Chem. Res. 21, 2238–2250.

    Article  CAS  Google Scholar 

  • Porchezhian, E., Ansari, S., 2001. Effect of Securigera securidaca on blood glucose levels of normal and alloxan-induced diabetic rats. Pharm. Biol. 39, 62–64.

    Article  Google Scholar 

  • Pushparaj, P., Tan, C., Tan, B., 2000. Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats. J. Ethnopharmacol. 72, 69–76.

    Article  CAS  PubMed  Google Scholar 

  • Sánchez-Rabaneda, F., Jáuregui, O., Casals, I., Andrés-Lacueva, C., Izquierdo-Pulido, M., Lamuela-Raventós, R.M., 2003. Liquid chromatographic/electrospray ionization tandem mass spectrometric study of the phenolic composition of cocoa) Theobroma cacao). J. Mass Spectrom. 38, 35–42.

    Article  PubMed  Google Scholar 

  • Sandhu, A., Gu, L., 2010. Antioxidant capacity, phenolic content, and profiling of phenolic compounds in the seeds, skin, and pulp of Vitis rotundifolia (muscadine grapes) as determined by HPLC-DAD-ESI-MSn. J. Agric. Food Chem. 58, 4681–4692.

    Article  CAS  PubMed  Google Scholar 

  • Siger, A., Nogala K, Lampart-Szczapa, E., 2008. The content and antioxidant activity of phenolic compounds in cold-pressed plant oils. J. Food Lipids. 15, 137–149.

    Article  CAS  Google Scholar 

  • Sun, J., Liang, F., Bin, Y., Li, P., Duan, C., 2007. Screening non-colored phenolics in red wines using liquid chromatography/ultraviolet and mass spectrometry/mass spectrometry libraries. Molecules 12, 679–693.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Trinder, P., 1969. Determination of blood glucose using 4-aminophenazone as oxygen acceptor. J. Clin. Pathol. 22, 246.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zahedi-Asl, S., Marahel, H., Zaree, B., 2005. Study on the effects of chloroformic extract of Securigera securidaca on serum glucose level and liver glycogen content of mice. J. Kerman Univ. Med. Sci. 12, 32–38.

    Google Scholar 

  • Zhang, M., Duan, C., Zang, Y., Huang, Z., Liu, G., 2011. The flavonoid composition of flavedo and juice from the pummelo cultivar (Citrus grandis(L.) Osbeck) and the grapefruit cultivar (Citrus paradisi) from China. Food Chem. 129, 1530–1536.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, 11562, Egypt

    Rana M. Ibrahim, Ali M. El-Halawany, Abd El-Rahman O. El-Shabrawy & Seham S. El-Hawary

  2. Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Ali M. El-Halawany

  3. Pharmacology Department, National Research Centre, Dokki, 12622, Cairo, Egypt

    Dalia O. Saleh

  4. Department of Pharmacognosy, Faculty of Pharmacy, Damanhour University, Damanhour City, Egypt

    El Moataz Bellah El Naggar

Authors
  1. Rana M. Ibrahim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ali M. El-Halawany
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dalia O. Saleh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. El Moataz Bellah El Naggar
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Abd El-Rahman O. El-Shabrawy
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Seham S. El-Hawary
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

RI wrote the manuscript, carried out extraction procedures and analyzed data. AM wrote the manuscript, planed the work and analyzed data. DS carried out biological activity. EN carried out LCMS analysis and interpreted data. AE revised the manuscript and supervised work. SE suggested the point and revised the manuscript.

Corresponding author

Correspondence to Ali M. El-Halawany.

Ethics declarations

The authors declare that they have no competing interests.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ibrahim, R.M., El-Halawany, A.M., Saleh, D.O. et al. HPLC-DAD-MS/MS profiling of phenolics from Securigera securidaca flowers and its anti-hyperglycemic and anti-hyperlipidemic activities. Rev. Bras. Farmacogn. 25, 134–141 (2015). https://doi.org/10.1016/j.bjp.2015.02.008

Download citation

  • Received: 22 December 2014

  • Accepted: 21 February 2015

  • Published: 23 March 2015

  • Issue Date: April 2015

  • DOI: https://doi.org/10.1016/j.bjp.2015.02.008

Share this article

Anyone you share the following link with will be able to read this content:

Sorry, a shareable link is not currently available for this article.

Provided by the Springer Nature SharedIt content-sharing initiative

Keywords

  • Securigera securidaca
  • Flowers
  • HPLC-DAD-MS/MS
  • Antidiabetic
  • Anti-hyperlipidemic
Use our pre-submission checklist

Avoid common mistakes on your manuscript.

Advertisement

Search

Navigation

  • Find a journal
  • Publish with us

Discover content

  • Journals A-Z
  • Books A-Z

Publish with us

  • Publish your research
  • Open access publishing

Products and services

  • Our products
  • Librarians
  • Societies
  • Partners and advertisers

Our imprints

  • Springer
  • Nature Portfolio
  • BMC
  • Palgrave Macmillan
  • Apress
  • Your US state privacy rights
  • Accessibility statement
  • Terms and conditions
  • Privacy policy
  • Help and support

Not affiliated

Springer Nature

© 2023 Springer Nature