Skip to main content
SpringerLink
Log in

Strontium ranelate treatment improves oxidative damage in osteoporotic rat model

  • Published:
Pharmacological Reports Aims and scope Submit manuscript

Abstract

Background

Osteoporosis is the most common skeletal disorder and is considered a risk of fracture. Most medication used for the treatment of osteoporosis is antiresorptive; however, strontium ranelate (Sr) therapy in postmenopausal women has shown a double effect on resorption and bone formation. In this study, the effect of Sr on status of the oxidative stress and antioxidant defence system was investigated.

Methods

Twenty-one adult albino female Wistar rats were used. The animals were randomly assigned into three groups, control (sham operated rats, received saline), OVX (ovariectomized rats), OVX + Sr (4 months later ovariectomy, strontium ranelate treatment was begun and continued for 120 days) each containing 7 animals. Strontium ranelate (500 mg/kg/day) and placebo (saline) were administered via oral gavage. At the end of the treatment, liver and kidney of rats were removed and malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were determined by biochemical analysis methods.

Results

In liver, MDAlevels were significantly higher in the OVX and OVX + Sr groups than the control group. GSH-Px activity decreased in OVX group and increased in OVX + Sr group compared with values of control group. CAT activity was increased in the OVX + Sr group when compared to control group. In kidney, MDAlevel was increased in OVX group. SOD activity was decreased in the OVX + Sr group. GSH-Px activity decreased in OVX group and increased in OVX + Sr group compared with control group. CAT activity increased in the OVX + Sr group when compared to control.

Conclusion

According to our results, Sr has preventive effect on oxidative damage in ovariectomized rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

CAT:

catalase

GSH-Px:

glutathione peroxidase

MDA:

malondialdehyde

NBT:

nitroblue tetrazolium

OVX:

ovariectomized

SOD:

superoxide dismutase

Sr:

strontium ranelate

References

  1. Aebi H: Catalase in vitro. Methods Enzymol, 1984, 105, 121–126.

    Article  CAS  Google Scholar 

  2. Banerjee BD, Seth V, Ahmed RS: Pesticide induced oxidative stress: perspectives and trends. Rev Environ Health, 2001, 16, 1–40.

    Article  CAS  Google Scholar 

  3. Blokhina O, Virolainen E, Fagerstedt KV: Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot, 2003, 91, 179–194.

    Article  CAS  Google Scholar 

  4. Borrás C, Sastre J, García-Sala D, Lloret A, Pallardó FV, Viña J: Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radic Biol Med, 2003, 34, 546–552.

    Article  Google Scholar 

  5. Canalis E, Hott M, Deloffre P, Tsouderos Y, Marie PJ: The divalent strontium salt S12911 enhances bone cell replication and bone formation in vitro. Bone, 1996, 18, 517–523.

    Article  CAS  Google Scholar 

  6. Folwarczna J, Zych M, Trzeciak HI: Effects of curcumin on the skeletal system in rats. Pharmacol Rep, 2010, 62, 900–909.

    Article  CAS  Google Scholar 

  7. Ha BJ: Oxidative stress in ovariectomy menopause and role of chondroitin sulfate. Arch Pharm Res, 2004, 27, 867–872.

    Article  CAS  Google Scholar 

  8. Hagihara M, Nishigaki I, Maseki M, Yagi K: Age-dependent changes in lipid peroxide levels in the lipoprotein fractions of human serum. J Gerontol, 1984, 39, 269–272.

    Article  CAS  Google Scholar 

  9. Kraenzlin ME, Anliker M, Griesmacher A: Laboratory assessment of osteoporosis (German). Ther Umsch, 2008, 65, 513–518.

    Article  Google Scholar 

  10. Konyalioglu S, Durmaz G, Yalcin A: The potential antioxidant effect of raloxifene treatment: a study on heart, liver and brain cortex of ovariectomized female rats. Cell Biochem Funct, 2007, 5, 259–266.

    Article  Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem, 1961, 193, 265–275.

    Google Scholar 

  12. Marie PJ, Hott M, Modrowski D, De Pollak C, Guillemain J, Deloffre P, Tsouderos Y: An uncoupling agent containing strontium prevents bone loss by depressing bone resorption and maintaining bone formation in estrogendeficient rats. J Bone Miner Res, 1993, 8, 607–615.

    Article  CAS  Google Scholar 

  13. Mates JM, Perez-Gomez C, De Castro IN: Antioxidant enzymes and human diseases. Clin Biochem, 1999, 32, 595–603.

    Article  CAS  Google Scholar 

  14. McCaslin FE Jr, James JM: The effect of strontium lactate in the treatment of osteoporosis. Proc Staff Meetings Mayo Clin, 1959, 34, 329–334.

    Google Scholar 

  15. Meunier PJ: The effects of strontium ranelate on the risk of vertebral fracture in women with postmenopausal osteoporosis. New Engl J Med, 2004, 350, 459–468.

    Article  CAS  Google Scholar 

  16. Muthusami S, Ramachandran I, Muthusamy B, Vasudevan G, Prabhu V, Subramaniam V, Jagadeesan A, Narasimhan S: Ovariectomy induces oxidative stress and impairs bone antioxidant system in adult rats. Clin Chim Acta, 2005, 360, 81–86.

    Article  CAS  Google Scholar 

  17. Oztekin E, Baltaci AK, Tiftik AM, Mogulkoc R: Lipid peroxidation in ovariectomized and pinealectomized rats: the effects of estradiol and progesterone supplementation. Cell Biochem Funct, 2007, 25, 551–554.

    Article  CAS  Google Scholar 

  18. Oztekin E, Mogulkoc R, Baltaci AK, Tiftik AM: The influence of estradiol and progesterone and melatonin supplementation on TNF-α levels in ovariectomized and pinealectomized rats. Acta Biol Hung, 2006, 57, 275–281.

    Article  Google Scholar 

  19. Oztekin E, Tiftik AM, Baltaci AK, Mogulkoc R: Lipid peroxidation in liver tissue of ovariectomized and pinealectomized rats: effect of estradiol and progesterone supplementation. Cell Biochem Funct, 2007, 25, 401–405.

    Article  CAS  Google Scholar 

  20. Paglia D, Valentine WN: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med, 1967, 70, 158–169.

    CAS  PubMed  Google Scholar 

  21. Reginster JY, Deroisy R, Jupsin I: Strontium ranelate: a new paradigm in the treatment of osteoporosis. Drugs Today, 2003, 39, 89–101.

    Article  CAS  Google Scholar 

  22. Reginster JY, Seeman E, De Vernejoul MC, Adami S, Compston J, Phenekos C, Devogelaer JP et al.: Strontium ranelate reduces the risk of nonvertebral fractures in postmenopausal women with osteoporosis: Treatment of Peripheral Osteoporosis (TROPOS) study. J Clin Endocrinol Metab, 2005, 90, 2816–2822.

    Article  CAS  Google Scholar 

  23. Shorr E, Carter AC: The usefulness of strontium as an adjuvant to calcium in the remineralization of the skeleton in man. Bull Hosp Joint Dis, 1952, 13, 59–66.

    CAS  PubMed  Google Scholar 

  24. Sun Y, Oberley LW, Ying L: A simple method for clinical assay of superoxide dismutase. Clin Chem, 1988, 34, 497–500.

    CAS  PubMed  Google Scholar 

  25. Verberckmoes SC, De Broe ME, D’Haese PC: Dosedependent effects of strontium on osteoblast function and mineralization. Kidney Int, 2003, 64, 534–543.

    Article  CAS  Google Scholar 

  26. Yagi K: Simple procedure for specific enzyme of lipid hydroperoxides in serum or plasma. Methods Mol Biol, 1998, 108, 107–110.

    CAS  PubMed  Google Scholar 

  27. Yalin S, Comelekoglu U, Bagis S, Sahin NO, Ogenler O, Hatungil R: Acute effect of single-dose cadmium treatment on lipid peroxidation and antioxidant enzymes in ovariectomized rats. Ecotoxicol Environ Saf, 2006, 65, 140–144.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Pharmacy Faculty, Mersin University, Yenisehir Campus, 33169, Mersin, Turkey

    Serap Yalin, Ozgun Sagir & Pelin Eroglu

  2. Department of Biophysics, Mersin University Medical School, Yenisehir Campus, 33169, Mersin, Turkey

    Ulku Comelekoglu

  3. Department of Pharmaceutical Technology, Pharmacy Faculty, Mersin University, Yenisehir Campus, 33169, Mersin, Turkey

    Mehmet Berköz

Authors
  1. Serap Yalin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ozgun Sagir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ulku Comelekoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mehmet Berköz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pelin Eroglu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Serap Yalin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yalin, S., Sagir, O., Comelekoglu, U. et al. Strontium ranelate treatment improves oxidative damage in osteoporotic rat model. Pharmacol. Rep 64, 396–402 (2012). https://doi.org/10.1016/S1734-1140(12)70780-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1734-1140(12)70780-6

Key words

Search

Navigation