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Anti-clastogenic potential of carnosic acid against 7,12-dimethylbenz(a) anthracene (DMBA)-induced clastogenesis

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Abstract

Carnosic acid, a primary phenolic compound found in the leaves of rosemary (Rosmarinus officinalis), has diverse pharmacological and biological activities. The aim of the present study was to investigate the anti-clastogenic effect of carnosic acid in DMBA-induced clastogenesis. The frequency of bone marrow micronucleated polychromatic erythrocytes (MnPCEs), chromosomal aberrations (cytogenetic end points), the status of Phase I and II detoxification enzymes, lipid peroxidation by-products and antioxidants (biochemical endpoints) were analyzed to assess the anti-clastogenic effect of carnosic acid in DMBA-induced clastogenesis. Oral pretreatment of carnosic acid for five days to DMBA-treated hamsters significantly protected DMBA-induced clastogenesis as well as biochemical abnormalities. Although the exact mechanism of anti-clastogenic effects of carnosic acid is unclear, the antioxidant potential and effect on modulation of Phase I and II detoxification enzymes could play a possible role.

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Abbreviations

CAT:

catalase

DMBA:

7,12-dimethylbenz(a)anthracene

GPx:

glutathione peroxidase

GR:

glutathione reductase

GSH:

reduced glutathione

GST:

glutathione-Stransferase

MnPCEs:

micronucleated polychromatic erythrocytes

NCEs:

normochromatic erythrocytes

PCEs:

polychromatic erythrocytes

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid reactive substances

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Correspondence to Shanmugam Manoharan.

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Manoharan, S., Balakrishnan, S., Vinothkumar, V. et al. Anti-clastogenic potential of carnosic acid against 7,12-dimethylbenz(a) anthracene (DMBA)-induced clastogenesis. Pharmacol. Rep 62, 1170–1177 (2010). https://doi.org/10.1016/S1734-1140(10)70379-0

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  • DOI: https://doi.org/10.1016/S1734-1140(10)70379-0

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