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Effect of platelet-activating factor antagonists (BN-52021, WEB-2170, and BB-882) on bacterial translocation in acute pancreatitis

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Journal of Gastrointestinal Surgery

Abstract

Bacterial translocation is an important source of pancreas infection in acute pancreatitis. The effect of platelet-activating factor (PAF) in the pathogenesis of acute pancreatitis has been proved in various studies. The aim of this study was to determine whether potent PAF antagonists influence bacterial translocation in acute pancreatitis. Acute pancreatitis was induced in 62 Wistar rats by injection of 2.5% sodium taurocholate into the biliopancreatic duct. The rats treated with PAF factor antagonists received intravenous injection of WEB-2170 (10 mg/kg), lexipafant (5 mg/kg), and BN-52021 (5 mg/kg) 30 minutes before induction of acute pancreatitis. Six hours after induction of acute pancreatitis, bacteriologic cultures and histologic scoring of tissues were performed. There was a statistically significant reduction in bacterial translocation to the mesenteric lymph nodes and liver but not to the pancreas of the rats treated with PAF antagonists. No significant increase in the intestinal bacterial population of any group was found. There were no statistical differences between the pancreatic histologic scores of the groups. PAF antagonists reduced bacterial translocation to distant sites other than the pancreas, preventing the bacterial dissemination that occurs in the early phase of acute pancreatitis and may have beneficial effects on the evolution of this disease.

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Authors and Affiliations

  1. Department of Gastroenterology, São Paulo University Medical School, São Paulo, Brazil

    Sandra Nassa Sampietre, Rosenilda Salvador Assis, Charles H. Knowles Ph.D., F.R.C.S., Kátia Ramos Leite M.D., Ph.D., Sonia Jancar Ph.D., José Eduardo Monteiro Cunha M.D., Ph.D. & Marcel Cerqueira Cesar Machado M.D., F.A.C.S.

  2. Rua Maria Jesus de Sirnões, 48 Lauzane Paulista, 02469-010, SāO Paulo SP, Brazil

    Lourenilson José de Souza M.D., Ph.D.

Authors
  1. Lourenilson José de Souza M.D., Ph.D.
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  2. Sandra Nassa Sampietre
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  3. Rosenilda Salvador Assis
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  4. Charles H. Knowles Ph.D., F.R.C.S.
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  8. Marcel Cerqueira Cesar Machado M.D., F.A.C.S.
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Supported by grant 97/02382-3 from the Fundação do Amparo a Pesquisa de São Paul0 (FAPESP).

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de Souza, L.J., Sampietre, S.N., Assis, R.S. et al. Effect of platelet-activating factor antagonists (BN-52021, WEB-2170, and BB-882) on bacterial translocation in acute pancreatitis. J Gastrointest Surg 5, 364–370 (2001). https://doi.org/10.1016/S1091-255X(01)80063-9

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  • DOI: https://doi.org/10.1016/S1091-255X(01)80063-9

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