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Interleukin-10 protects against lethalit of intra- abdominal infection and sepsis

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Journal of Gastrointestinal Surgery

Abstract

The aim of this study was to determine whether interleukin-10 would alter locally derived and systemic proinflammatory cytokine expression and protect from the lethality of cecal ligation and puncture. Three groups of Sprague-Dawley rats were used. Group 1 underwent cecal manipulation. Groups 2 and 3 underwent cecal ligation and puncture. Group 2 received intraperitoneal saline injections beginning 1 hour after cecal ligation and puncture and every 3 hours thereafter for 24 hours. Group 3 received intraperitoneal interleukin-10 one hour after cecal ligation and puncture and every 3 hours thereafter. Animals were killed at 6 and 24 hours after cecal ligation and puncture or sham operation. Serum tumor necrosis factor-alpha (TNF-α) levels were determined by enzyme-linked immunosorhent assay. TNF-α messenger RNA expression was determined by reverse transcriptase–polymerase chain reaction using β-actin as the internal standard. There was a twofold increase (P <0.001) in TNF-α mRNA in the liver at 6 and 24 hours after cecal ligation and puncture when compared to rats treated with interleukin-10. There was a twofold increase (P <0.05) in TNF-α mRNA in the lung observed only at 24 hours after cecal ligation and puncture when compared to rats treated with interleukin-10. Serum levels of TNF-α were elevated at 6 hours in control animals, and this effect was abolished by the administration of interleukin-10. There was no difference in mortality rates at 6 hours (0% for all groups); however, at 24 hours 57% (4/7) mortality was observed in group 2 vs. 0% (0/20) in groups 1 and 3. Interleukin-10 given after the onset of cecal ligation and puncture protects against the lethality of intra-abdominal sepsis.

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Authors and Affiliations

  1. Departments of Surgery, UCLA and Sepulveda VA Medical Centers, Los Angeles, Calif.

    Anthony J. Rongione M.D., Amy M. Kusske M.D., Karen Kwan B.S., Stanley W. Ashley M.D., Howard A. Reber M.D. & David W. McFadden M.D.

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  1. Anthony J. Rongione M.D.
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  2. Amy M. Kusske M.D.
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  4. Stanley W. Ashley M.D.
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Supported by Veterans Administration Merit Review Funds (H.A.R., S.W.A., and D.W.M).

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Rongione, A.J., Kusske, A.M., Kwan, K. et al. Interleukin-10 protects against lethalit of intra- abdominal infection and sepsis. J Gastrointest Surg 4, 70–76 (2000). https://doi.org/10.1016/S1091-255X(00)80035-9

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  • DOI: https://doi.org/10.1016/S1091-255X(00)80035-9

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