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Inflammatory Cytokine mRNA in Human Gestational Tissues: Implications for Term and Preterm Labor

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The Journal of the Society for Gynecologic Investigation: JSGI Aims and scope Submit manuscript

Abstract

Objective

To determine if inflammatory cytokine mRNA in gestational tissues is present only in the setting of infection-associated preterm labor or under several other clinical conditions.

Methods

Human gestational tissues were collected from 51 women experiencing 1) term cesarean delivery without labor, 2) normal term vaginal delivery, 3) preterm cesarean delivery without labor, 4) preterm vaginal delivery without chorioamnionitis, and 5) preterm vaginal delivery with concomitant chorioamnionitis. Decidua, chorion, and amnion were isolated, total RNA from each tissue was extracted, and the presence of inflammatory cytokine mRNA was determined by polymerase chain reaction. Intcrleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α mRNA was detected using specific oligonucleotide primers.

Results

Interleukin-1β mRNA was rarely found in tissues preterm without labor but was readily detected in both maternal and fetal tissues after labor, regardless of gestational age. Interleukin-6 mRNA was rare in tissues from the nonlaboring patient but was found in almost all tissues after labor. Interleukin-8 mRNA was detected in all tissues at term, both in nonlaboring and laboring patients. Tumor necrosis factor-α mRNA was detected in only 20–50% of tissues after labor, and was rarely detected in the absence of labor.

Conclusions

Inflammatory cytokine mRNA is commonly expressed in human gestational tissues after normal labor and preterm labor with or without associated intrauterine infection. There was no difference in the pattern of expression of mRNA inflammatory cytokine in women who did or did not have clinically evident intrauterine infection.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, and Pediatrics, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, Utah, 84132, USA

    Donald J. Dudley MD, Deborah Collmer BS, Murray D. Mitchell DPhil & Michael S. Trautman MD

  2. Department of Pharmacology and Clinical Pharmacology, University of Auckland, Auckland, New Zealand

    Donald J. Dudley MD, Deborah Collmer BS, Murray D. Mitchell DPhil & Michael S. Trautman MD

Authors
  1. Donald J. Dudley MD
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  2. Deborah Collmer BS
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  3. Murray D. Mitchell DPhil
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  4. Michael S. Trautman MD
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Additional information

Supported by National Institutes of Health grant K08-HD00964-03 (DJD).

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Dudley, D.J., Collmer, D., Mitchell, M.D. et al. Inflammatory Cytokine mRNA in Human Gestational Tissues: Implications for Term and Preterm Labor. Reprod. Sci. 3, 328–335 (1996). https://doi.org/10.1016/S1071-5576(96)00042-1

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  • DOI: https://doi.org/10.1016/S1071-5576(96)00042-1

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