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Neuropeptide Y and Calcitonin Gene-Related Peptide Modulate Voltage-Gated Ca2+ Channels in Mature Female Rat Paracervical Ganglion Neurons

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Abstract

Objective

To assess the predominant subtype of calcium channel present in neurons of the paracervical ganglia (PG) of the female rat and the ability of neuroactive peptides to modulate total calcium current.

Methods

Whole-cell patch clamp techniques were used on isolated PG neurons to assess calcium current modulation in the presence and absence of selective calcium channel subtype inhibitors and neuropeptides. Digital imaging analysis of cells was used to determine neuronal cell size distributions within the ganglia.

Results

Average PG cell diameter was 28.1 μm. Most PG neurons (64%) had an N-type calcium current that contributed 41% of the total calcium current. The low voltage-activated or T-type current was not present. Very few neurons (22%) were sensitive to the P-type calcium channel blocker ω-agatoxin IVA, and in only 10% of neurons was the calcium current sensitive to the L-type channel blocker nimodipine. Neuropeptide Y (NPY) inhibited the calcium current by 41% in 79% of the neurons, but vasoactive intestinal peptide (VIP) had no effect. Calcitonin gene-related peptide (CGRP) both increased and decreased the calcium current in separate cell populations.

Conclusions

Calcium currents in female rat PG neurons are carried primarily through N-type calcium channels with a small contribution from L- and P-type channels. An unidentified calcium channel type is also present. Modulation of the calcium current by NPY is demonstrated, and support for the presence of a local, CGRP-mediated circuit is presented.

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Authors and Affiliations

  1. Departments of Obstetrics and Gynecology and Pharmacology and Toxicology, Augusta, Georgia, USA

    David P. Cohen MD, Stephen R. Ikeda MD, PhD & Deborah L. Lewis PhD

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  1. David P. Cohen MD
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  2. Stephen R. Ikeda MD, PhD
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  3. Deborah L. Lewis PhD
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Additional information

This work was supported by a grant from the National Institute of Neurological Disorders and Stroke, National Institutes of Health NS28894 (DLL). The authors thank E. Zboran for excellent technical assistance, Dr. R. E. Papka for mitial discussion, and Drs. P. McDonough, L. Plouffe, Jr, and K. Hansen for their critical review of the manuscript.

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Cohen, D.P., Ikeda, S.R. & Lewis, D.L. Neuropeptide Y and Calcitonin Gene-Related Peptide Modulate Voltage-Gated Ca2+ Channels in Mature Female Rat Paracervical Ganglion Neurons. Reprod. Sci. 3, 342–349 (1996). https://doi.org/10.1016/S1071-5576(96)00041-X

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