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Secondary ion images of the rodent brain

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Journal of the American Society for Mass Spectrometry

Abstract

Sections of biologic tissue obtained from laboratory rodents are prepared and analyzed by secondary ion mass spectrometry. The intensity of phosphocholine secondary ions is used to identify anatomical features of the brain from secondary ion images and to evaluate the effectiveness of procedures developed. Secondary ion emission of phosphocholine (m/z 184), is found to be abundant and its intensity is heterogeneous. Effects of sample thickness are addressed. Correspondence between conventional optical images of stained tissue and secondary ion images shows that successive ion images may be used to produce a three-dimensional map of the brain, i.e., an atlas.

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Authors and Affiliations

  1. Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

    C. A. McCandlish, J. M. McMahon & P. J. Todd

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  1. C. A. McCandlish
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  2. J. M. McMahon
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  3. P. J. Todd
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Correspondence to P. J. Todd.

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McCandlish, C.A., McMahon, J.M. & Todd, P.J. Secondary ion images of the rodent brain. J Am Soc Mass Spectrom 11, 191–199 (2000). https://doi.org/10.1016/S1044-0305(99)00145-2

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  • DOI: https://doi.org/10.1016/S1044-0305(99)00145-2

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