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Mechanistic studies of multipole storage assisted dissociation

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Journal of the American Society for Mass Spectrometry

Abstract

The degree and onset of fragmentation in multipole storage assisted dissociation (MSAD) have been investigated as functions of several hexapole parameters. Strict studies of hexapole charge density (number of ions injected) and hexapole storage time were made possible by placing a pulsed shutter in front of the entrance to the mass spectrometer. The results obtained show that the charge density is the most critical parameter, but also dependencies on storage time, radio-frequency (rf) -amplitude, and pressure are seen. From these data, and from simulations of the ion trajectories inside the hexapole, a mechanism for MSAD, similar to the ones for sustained off-resonance irradiation (SORI), and for low energy collisionally induced dissociation in the collision multipole of a triple quadrupole mass spectrometer, is proposed. It is believed that, at higher charge densities, ions are pushed to larger hexapole radii where the electric potential created by the rf field is higher, forcing the ions to oscillate radially to higher amplitudes and thereby reach higher (but still relatively low) kinetic energies. Multiple collisions with residual gas molecules at these elevated energies then heat up the molecules to their dissociation threshold. Further support for this mechanism is obtained from a comparison of MSAD and SORI spectra which are almost identical in appearance.

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Author notes

  1. Jan Axelsson

    Present address: Amersham Pharmacia Biotech, SE-751 84, Uppsala, Sweden

Authors and Affiliations

  1. Ion Physics Division, Ångström Laboratory, Uppsala University, Box 534, SE-751 21, Uppsala, Sweden

    Kristina Håkansson, Jan Axelsson, Magnus Palmblad & Per Håkansson

Authors
  1. Kristina Håkansson
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  2. Jan Axelsson
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  3. Magnus Palmblad
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  4. Per Håkansson
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Correspondence to Kristina Håkansson.

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Håkansson, K., Axelsson, J., Palmblad, M. et al. Mechanistic studies of multipole storage assisted dissociation. J Am Soc Mass Spectrom 11, 210–217 (2000). https://doi.org/10.1016/S1044-0305(99)00144-0

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  • DOI: https://doi.org/10.1016/S1044-0305(99)00144-0

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