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Rapid confirmation/quantitation of cocaine and benzoylecgonine in urine utilizing high performance liquid chromatography and tandem mass spectrometry

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Journal of the American Society for Mass Spectrometry

Abstract

A rapid, but sensitive and selective method for confirmation and quantitation of benzoylecgonine (BZE) and cocaine (COC) in urine by fast-gradient liquid chromatography/tandem mass spectrometry (LC/MS/MS) is described. The chromatographic separation was performed on a reversed phase column employing fast-gradient techniques. Matrix prepared standards, blanks, and QC’s were filtered then aliquots were transferred into a 96 well plate. Injection volumes of 25 μL were made onto the analytical column, with the flow diverted from the atmospheric pressure ionization source for the first 0.5 min of the analysis. Simultaneous multiple reaction monitoring (MRM) of three discrete transitions for each compound were used to identify BZE and COC. Quantitation was achieved utilizing the most prominent parent—daughter transition and internal standard calibration techniques. The coefficients of variation (CV) for the analysis of these drugs ranged from 0.6% to 6.8% at a concentration of 150 ng/mL (n = 155). This method suggests that fast-gradient LC/MS/MS may be suitable for routine confirmation of immunoassay cocaine-positive samples.

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Authors and Affiliations

  1. Drug Metabolism Technology Department, Pfizer Inc., Central Research Division, Eastern Point Road, Bldg. 118N, N212, Box 8003, 06340, Groton, CT

    Patrick M. Jeanville, Shane R. Needham & Mark J. Cole

  2. College of Health-Related Professions, University of Puerto Rico, Medical Sciences Campus, San Juan, Puerto Rico

    Estela S. Estapé

Authors
  1. Patrick M. Jeanville
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  2. Estela S. Estapé
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  3. Shane R. Needham
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  4. Mark J. Cole
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Correspondence to Patrick M. Jeanville.

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Jeanville, P.M., Estapé, E.S., Needham, S.R. et al. Rapid confirmation/quantitation of cocaine and benzoylecgonine in urine utilizing high performance liquid chromatography and tandem mass spectrometry. J Am Soc Mass Spectrom 11, 257–263 (2000). https://doi.org/10.1016/S1044-0305(99)00138-5

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  • DOI: https://doi.org/10.1016/S1044-0305(99)00138-5

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