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Separation and detection of the α- and β-chains of hemoglobin of a single intact red blood cell using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry

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Journal of the American Society for Mass Spectrometry

Abstract

A single intact red blood cell (erythrocyte) was injected into a capillary electrophoresis column, and following in-capillary lysing the α- and β-chains of the hemoglobin (∼450 amol) were separated and detected using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry. The mass spectra of the electropherogram peaks of the α and β chains showed identifiable peaks corresponding to multiply protonated and sodiated α- and β-chains of hemoglobin.

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Author notes

  1. Ping Cao

    Present address: Genentech, Inc., 94080, South San Francisco, CA

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Texas at Austin, 78712, Austin, TX

    Ping Cao & Mehdi Moini

Authors
  1. Ping Cao
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  2. Mehdi Moini
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Correspondence to Mehdi Moini.

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Cao, P., Moini, M. Separation and detection of the α- and β-chains of hemoglobin of a single intact red blood cell using capillary electrophoresis/electrospray ionization time-of-flight mass spectrometry. J Am Soc Mass Spectrom 10, 184–186 (1999). https://doi.org/10.1016/S1044-0305(98)00146-9

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00146-9

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