Abstract
The potential of matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay (PSD) time-of-flight mass spectrometry for the characterization of peptides and proteins is discussed. Recent instrumental developments provide for levels of sensitivity and accuracy that make these techniques major analytical tools for proteome analysis. New software developments employing protein database searches have greatly enhanced the fields of application of MALDI-PSD. Peptides and proteins can be easily identified even if only a partial sequence information is determined. Derivatization procedures have been optimized for MALDI-PSD to increase the structural information and to obtain a complete peptide sequence even in critical cases. They are fast, simple and can be performed on target. MALDI-PSD is also a very powerful tool to characterize or elucidate post-translational or chemically induced modifications. In association with database searches, proteins issued from electrophoretic gels can be identified after specific enzymatic cleavages and peptide mapping.
Similar content being viewed by others
References
Burlingame, A. L.; Boyd, R. K.; Gaskell, S. J. Anal. Chem. 1996, 68, 599R-651R.
Roepstorff, P. Curr. Opin. Biotechnol. 1997, 8, 6–13.
Yates, J. R. J. Mass. Spectrom. 1998, 33, 1–19.
Karas, M.; Hillemkamp, F. Anal. Chem. 1988, 60, 2299–2301.
Hillenkamp, F.; Karas, M.; Beavis, R. C.; Chait, B. T. Anal. Chem. 1991, 63, 1193A-1203A.
Burlingale, A. L.; Boyd, R. K.; Maltby, D. M. Anal. Chem. 1994, 66, 634R-683R.
Kaufmann, R. J. Biotechnol. 1995, 41, 155–175.
Mann, M.; Talbo, G. Curr. Opinion Biotechnol. 1996, 7, 11–19.
Special issue: Matrix-assisted laser desorption/ionisation mass spectrometry. Int. J. Mass Spectrom. Ion. Processes. 1997, 169/170, vii–viii.
Brown, R. S.; Lennon, J. J. Anal. Chem. 1995, 67, 1998–2003.
Colby, S. M.; King, T. B.; Reilly, J. P. Rapid Commun. Mass Spectrom. 1994, 8, 865–868.
Whittal, R. M.; Li, L. Anal. Chem. 1995, 67, 1950–1954.
Juhasz, P.; Vestal, M. L.; Martin, S. A. J. Am. Mass Spectrom. 1997, 8, 209–217.
Vestal, M. L.; Juhasz, P.; Martin, S. A. Rapid Commun. Mass Spectrom. 1995, 9, 1044–1050.
Russel, D. H.; Edmondson, R. D. J. Mass Spectrom. 1997, 32, 263–276.
Takach, E. J.; Hines, W. M.; Patterson, D. H.; Juhasz, P.; Falick, A. M.; Vestal, M. L.; Martin, S. A. J. Prot. Chem. 1997, 16, 363–369.
Spengler, B.; Kirsch, D.; Kaufmann, R.; Jaeger, E. Rapid Commun. Mass Spectrom. 1992, 6, 105–108.
Kaufmann, R.; Spengler, B.; Lützenkirchen, F. Rapid Commun. Mass Spectrom. 1993, 7, 902–910.
Rouse, J. C.; Yu, W.; Martin, S. A. J. Am. Mass Spectrom. 1995, 6, 822–835.
Jai-nhuknan, J.; Cassady, C. J. Rapid Commun. Mass Spectrom. 1996, 10, 1678–1682.
Stimson, E.; Truong, O.; Richter, W. J.; Waterfield, M. D.; Burlingame, A. L. Int. J. Mass Spectrom. Ion Processes 1997, 169/170, 231–240.
Stahl-Zeng, J.; Hillenkamp, F. Eur. Mass Spectrom. 1996, 2, 23–32.
Cornish, T. J.; Cotter, R. J. Rapid Commun. Mass Spectrom. 1994, 8, 781–785.
Kaufmann, R.; Chaurand, P.; Kirsch, D.; Spengler, B. Rapid Commun. Mass Spectrom. 1996, 10, 1199–1208.
Spengler, B.; Bold, P.; Chaurand, P.; Lützenkirchen, F.; Metzger, S.; Kaufmann, R. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics.; June 1–5, 1997; p 1103.
Spengler, B. J. Mass Spectrom. 1997, 38, 1019–1036.
Ambihapathy, K.; Yalcin, T.; Leung, H. W.; Harrison, A. G. J. Mass Spectrom. 1997, 32, 209–215.
Kaufmann, R.; Kirsch, D.; Spengler, B. Int. J. Mass Spectrom. Ion Process. 1994, 131, 355–385.
Yalcin, T.; Khouw, C.; Csizmadia, I. G.; Peterson, M. R.; Harrison, A. G. J. Am. Mass Spectrom. 1995, 6, 1165–1174.
Yalcin, T.; Csizmadia, I. G.; Peterson, M. R.; Harrison, A. G. J. Am. Mass Spectrom. 1996, 7, 233–242.
Biemann, K. Methods Enzymol 1990, 193, 455–479.
Roepstorff, P.; Fohlmann, J. Biomed. Mass Spectrom. 1984, 11(11), 601.
Johnson, R. S.; Martin, R. S.; Biemann, K. Int. J. Mass Spectrom. Ion Process. 1988, 86, 137–154.
Lützenkirchen, F.; Chaurand, P.; Spengler, B.; Kaufmann, R. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics, June 1–5, 1997; p 1001.
Mann, M.; Wilm, M. Anal. Chem. 1994, 66, 4390–4399.
http://www.mann.embl-heidelberg.de
Epstein, L. B.; Smith, D. M.; Matsui, N. M.; Tran, H. M.; Sullivan, C.; Raineri, I.; Burlingame, A. L.; Clauser, K. R.; Hall, S. C.; Andrews, L. E. Electrophoresis 1996, 17, 1655–1670.
Sullivan, C. M.; Smith, D. M.; Matsui, N. M.; Andrews, L. E.; Clauser, K. R.; Chapeaurouge, A.; Burlingame, A. L.; Epstein, L. B. Cancer Res 1997, 57, 1137–1143.
Gevaert, K.; Demol, H.; Skylyarova, T.; Vandekerckhove, J.; Houthave, T. Electrophoresis 1998, 19, 909–917.
Flad, Th.; Spengler, B.; Kalbacher, H.; Brossart, P.; Baier, D.; Kaufmann, R.; Bold, P.; Metzger, S.; Meyer, H.; Kurz, B.; Mueller, C. A. Cancer Res. (in press).
Lützenkirchen, F.; Chaurand, P.; Spengler, B.; Kaufmann, R. Proceedings of the 14th IMSC Conference; Tampere, Finland; August, 25–29, 1997.
Spengler, B.; Lützenkirchen, F.; Kaufmann, R. Organ. Mass Spectrom. 1993, 28, 1482–1490.
Chaurand, P.; Kaufmann, R.; Lützenkirchen, F.; Spengler, B.; Fournier, I.; Brunot, A.; Blais, J. C.; Tabet, J. C.; Bolbach, G. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics; June 1–5, 1997; p 1337.
Hunt, D. F.; Buko, A. M.; Ballard, J. M.; Shabanowitz, J.; Giordani, A. B. Biomed. Mass Spectrom. 1981, 8, 397–417.
Huang, Z. H.; Wu, J.; Roth, K. D. W.; Yang, Y.; Gage, D. A.; Watson, J. T. Anal. Chem. 1997, 69, 137–144.
Spengler, B.; Lützenkirchen, F.; Metzger, S.; Chaurand, P.; Kaufmann, R.; Jeffery, W.; Bartlet-Jones, M.; Pappin, D. J. C. Int. J. Mass Spectrom. Ion Process. 1997, 169/170, 277–290.
Kaufmann, R.; Kirsch, D.; Tourmann, J. L.; Machold, J.; Hucho, F.; Utkin, Yu.; Tsetlin, V. Eur. Mass Spectrom. 1995, 1, 313–325.
Melnyk, O.; Chaurand, P.; Rommens, C.; Drobecq, H.; Wieruszeski, J. M.; Spengler, B.; Gras-Masse, H. J. Peptide Res. 1998, 51, 188–193.
Tsarbopoulos, A.; Bahr, U.; Pramanik, B. N.; Karas, M. Int. J. Mass Spectrom. Ion Process. 1997, 169/170, 251–261.
Annan, R. S.; Carr, S. A. Anal. Chem. 1996, 68, 3413–3421.
Schnölzer, M.; Lehmann, W. D. Int. J. Mass Spectrom. Ion Process. 1997, 169/170, 263–271.
Jespersen, S.; Chaurand, P.; Van Strien, F. J. C.; Spengler, B.; Kaufmann, R.; Van Der Greef, J. Anal. Chem. (unpublished).
Patterson, S. D.; Aebersold, R. Electrophoresis 1995, 16, 1791–1814.
Elicone, C.; Lui, M.; Geromanos, S.; Erdjument-Bromage, H.; Tempst, P. J. Chromatogr. 1994, 676, 121–137.
Tempst, P.; Erdjument-Bromage, H.; Casteels, P.; Geromanos, S.; Lui, M.; Powel, M.; Nelson, R. W. In Mass Spectrometry in the Biological Sciences; Burlingame, A. L.; Carr, S. A., Eds.; Humana, Totowa, NJ, 1996; pp 105–133.
Zhang, H.; Caprioli, R. M. J. Mass Spectrom. 1996, 31, 1039–1046.
Hutchens, T. W.; Yip, T. T. Rapid Commun. Mass Spectrom. 1993, 7, 576–580.
Nakanishi, T.; Okamoto, N.; Tanaka, K.; Shimizu, A. Biol. Mass Spectrom. 1994, 23, 230–233.
Phizichy, E. M.; Fields, S. Microbiol. Rev. 1995, 59, 94–123.
Mann, M.; Hojrup, P.; Roepstorff, P. Biol. Mass Spectrom. 1993, 22, 338–345.
Henzel, W. J.; Billeci, T. M.; Stults, J. T.; Wong, S. C. Proc. Natl. Acad. Sci. (USA) 1993, 90, 5011–5015.
Pappin, D. J. C.; Hojrup, P.; Bleasby, A. J. Curr. Biol. 1993, 3, 327–332.
James, P. M.; Quadroni, J. D. C.; Carafoli, E.; Gonnet, G. Biophys. Biochem. Res. Commun. 1993, 195, 58–64.
Yates, J. R.; Speicher, S.; Griffin, P. R.; Hunkapiller, T. Anal. Biochem. 1993, 214, 397–408.
Shevchenko, A.; Wilm, M.; Vorm, O.; Jensen, O. M.; Potelejnikov, A.; Neubauer, G.; Shevchenko, A.; Mortensen, P.; Mann, M. Biochem. Soc. Trans. 1996, 28, 893.
Patterson, S. D.; Aebersold, R. Electrophoresis 1995, 16, 1791–1814.
Jensen, O. N.; Potelejnikov, A.; Mann, M. Rapid Commun. Mass Spectrom. 1996, 10, 1371–1378.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chaurand, P., Luetzenkirchen, F. & Spengler, B. Peptide and protein identification by matrix-assisted laser desorption ionization (MALDI) and MALDI-post-source decay time-of-flight mass spectrometry. J Am Soc Mass Spectrom 10, 91–103 (1999). https://doi.org/10.1016/S1044-0305(98)00145-7
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/S1044-0305(98)00145-7