Skip to main content
SpringerLink
Log in

Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument

  • Short Communication
  • Published:
Journal of the American Society for Mass Spectrometry

Abstract

The automated use of a matrix-assisted laser desorption ionization (MALDI) mass spectrometer (MS) is described for image analysis of samples through implementation of new software for instrument control, data acquisition, and data analysis. The software permits automated acquisition of MS MALDI spectra to form an ordered data array and contains display features to provide images at one or more mass-to-charge ratio values. The technique can be used to scan tissue samples, blotted samples, gels, or other sample surfaces where the image analysis of that sample is required. The program achieves a time of typically 1 s per image point, permitting an analysis made up of large numbers of points with high spatial resolution up to 850 dpi. The features of the software are demonstrated in this paper with samples of printed images, where visible images can be compared to those obtained by mass spectrometry. Quantitative aspects are introduced by analyzing a series of sample spots containing different amounts of several proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Briggs, D.; Seah, M. P. Practical Surface Analysis; Wiley: New York, 1996, Vol. 2 367–424.

    Google Scholar 

  2. VanHoolst, W. K.; VanEspen, P. J. Image Processing in Secondary Ion Mass Spectrometry. Mikrochim. Acta 1991, 2, 415–425.

    Article  CAS  Google Scholar 

  3. Mony, M. C.; Larras-Regard, E. Imaging of subcellular structures by scanning ion microscopy and mass spectrometry. Biol. Cell. 1997, 89, 199–210.

    Article  CAS  Google Scholar 

  4. Colliver, T. L.; Brummel, C. L.; Pacholski, M. L.; Swanek, F. D.; Ewing, A. G.; Winograd, N. Atomic and molecular imaging at the single-cell level with TOF-SIMS. Anal. Chem. 1997, 69, 2225–2231.

    Article  CAS  Google Scholar 

  5. McMahon, J. M.; Short, R. T.; McCandlish, C. A.; Brenna, J. T.; Todd, P. J. Identification and mapping of phosphocholine in animal tissue by static secondary ion mass spectrometry and tandem mass spectrometry. Rapid Commun. Mass Spectrom. 1996, 10, 335–340.

    Article  CAS  Google Scholar 

  6. Van Vaeck, L.; Struyf, H.; Van Roy, W.; Adams, F. Organic and Inorganic Analysis with Laser Microprobe Mass Spectrometry Part 1: Instrumentation and Methodology. Mass Spec. Rev. 1994, 13, 189–208.

    Article  CAS  Google Scholar 

  7. Van Vaeck, L.; Struyf, H.; Van Roy, W.; Adams, F. Organic and Inorganic Analysis with Laser Microprobe Mass Spectrometry Part 2: Applications. Mass Spec. Rev. 1994, 13, 209–232.

    Article  CAS  Google Scholar 

  8. Van Vaeck, L.; Poels, K.; De Nollin, S.; Hachimi, A.; Gijbels, R. Laser microprobe mass spectrometry: principle and applications in biology and medicine. Cell Biol. Int. 1997, 21, 635–648.

    Article  Google Scholar 

  9. Iancu, T. C.; Perl, D. P.; Sternlieb, I.; Lerner, A.; Leshinsky, E.; Kolodny, E. H.; Hsu, A.; Good, P. F. The application of laser microprobe mass analysis to the study of biological material. Biometals 1996, 9, 57–65.

    Article  CAS  Google Scholar 

  10. Pestaner, J. P.; Mullick, F. G.; Centeno, J. A. Characterization of acetaminophen: molecular microanalysis with Raman microprobe spectroscopy. J. Forensic Sci. 1996, 41, 1060–1063.

    CAS  Google Scholar 

  11. Huber, M.; Spengler, B.; Kaufmann, R. Development of a new Scanning UV-Laser Microprobe for Ion Imaging and Confocal Microscopy. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics; Chicago, Illinois, 1994; p 1044.

  12. Caprioli, R. M.; Farmer, T. B.; Gile, J. Molecular Imaging of Biological Samples: Localization of Peptides and Proteins Using MALDI-TOF MS. Anal. Chem. 1997, 69, 4751–4760.

    Article  CAS  Google Scholar 

  13. Haiying, Z. Development of Combined Micro-Preparation, Separation and Mass Spectrometry Methods and Applications in the Microdialysis Study of Neuropeptides. Ph.D. Thesis, University of Texas Health Science Center, Houston, 1998.

    Google Scholar 

  14. Schreiner, M.; Strupat, K.; Lottspeich, F.; Eckerskorn, C. Ultraviolet matrix assisted laser desorption ionization-mass spectrometry of electroblotted proteins. Electrophoresis 1996, 17, 954–961.

    Article  CAS  Google Scholar 

  15. Stahl, B.; Linos, A.; Karas, M.; Hillenkamp, F. Setup M Analysis of fructans from higher plants by matrix-assisted laser desorption/ionization mass spectrometry. Anal. Biochem. 1997, 246, 195–204.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Mass Spectrometry Research Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA

    Markus Stoeckli, Terry B. Farmer & Richard M. Caprioli

Authors
  1. Markus Stoeckli
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Terry B. Farmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard M. Caprioli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Richard M. Caprioli.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stoeckli, M., Farmer, T.B. & Caprioli, R.M. Automated mass spectrometry imaging with a matrix-assisted laser desorption ionization time-of-flight instrument. J Am Soc Mass Spectrom 10, 67–71 (1999). https://doi.org/10.1016/S1044-0305(98)00126-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1044-0305(98)00126-3

Keywords

Search

Navigation