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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for polymer analysis: solvent effect in sample preparation

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Journal of the American Society for Mass Spectrometry

Abstract

The success of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry for the characterization of polymer structures and for the determination of average molecular weights and distributions depends on the use of a proper sample/matrix preparation protocol. This work examines the effect of solvents, particularly solvent mixtures, used to prepare polymer, matrix, and cationization reagent solutions, on MALDI analysis. It is shown that the use of solvent mixtures consisting of polymer solvents does not have a significant effect on the molecular weight determination of polystyrene 7000 and poly(methyl methacrylate) 3750. However, solvent mixtures containing a polymer nonsolvent can affect the signal reproducibility and cause errors in average weight measurement. This solvent effect was further investigated by using confocal laser fluorescence microscopy in conjunction with the use of a fluorescein-labeled polystyrene. It is demonstrated that sample morphology and polymer distribution on the probe can be greatly influenced by the type of solvents used. For sample preparation in MALDI analysis of polymers, it is important to select a solvent system that will allow matrix crystallization to take place prior to polymer precipitation. The use of an excess amount of any polymer nonsolvent should be avoided.

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References

  1. Bahr, U.; Deppe, A.; Karas, M.; Hillenkamp, F. Anal. Chem. 1992, 64, 2866–2869.

    Article  CAS  Google Scholar 

  2. Danis, P. O.; Karr, D. E. Org. Mass Spectrom. 1993, 28, 923–925.

    Article  CAS  Google Scholar 

  3. Lee, S.; Winnik, M. A.; Whittal, R. M.; Li, L. Macromolecules 1996, 29, 3060–3072.

    Article  CAS  Google Scholar 

  4. Jackson, A. T.; Yates, H. T.; Scrivens, J. H.; Critchley, G.; Brown, J.; Green, M. R.; Bateman, R. H. Rapid Commun. Mass Spectrom. 1996, 10, 1668–1674.

    Article  CAS  Google Scholar 

  5. Weidner, S.; Kuhn, G.; Just, U. Rapid Commun. Mass Spectrom. 1995, 9, 697–702.

    Article  CAS  Google Scholar 

  6. Danis, P. O.; Karr, D. E.; Westmoreland, D. G.; Piton, M. C.; Christie, D. I.; Clay, P. A.; Kable, S. H.; Gilbert, R. G. Macromolecules 1993, 26, 6684–6685.

    Article  CAS  Google Scholar 

  7. Wilczek-Vera, G.; Danis, P. O.; Eisenberg, A. Macromolecules 1996, 29, 4036–4044.

    Article  CAS  Google Scholar 

  8. Montaudo, G.; Montaudo, M. S.; Puglisi, C.; Samperi, F. Macromolecules 1995, 28, 4562–4569.

    Article  CAS  Google Scholar 

  9. Lloyd, P. M.; Suddaby, K. G.; Varney, J. E.; Scrivener, E.; Derrick, P. J.; Haddleton, D. M. Eur. Mass. Spectrom. 1995, 1, 293–300.

    Article  CAS  Google Scholar 

  10. Belu, A. M.; DeSimone, J. M.; Linton, R. W.; Lange, G. W.; Friedman, R. M. J. Am. Soc. Mass Spectrom. 1996, 7, 11–24.

    Article  CAS  Google Scholar 

  11. Jackson, C.; Larsen, B.; McEwen, C. Anal. Chem. 1996, 68, 1303–1308.

    Article  CAS  Google Scholar 

  12. Danis, P. O.; Karr, D. E.; Xiong, Y.; Owens, K. G. Rapid Commun. Mass Spectrom. 1996, 10, 862–868.

    Article  CAS  Google Scholar 

  13. Schriemer, D. C.; Li, L. Anal. Chem. 1996, 68, 2721–2725.

    Article  CAS  Google Scholar 

  14. Zhou, H. H.; Yalcin, T.; Li, L. J. Am. Soc. Mass Spectrom. 1998, 9, 275–281.

    Article  Google Scholar 

  15. Whittal, R. M.; Li, L.; Lee, S.; Winnik, M. A. Macromol. Rapid Commun. 1996, 17, 59–64.

    Article  CAS  Google Scholar 

  16. Schriemer, D. C.; Whittal, R. M.; Li, L. Macromolecules 1997, 30, 1955–1963.

    Article  CAS  Google Scholar 

  17. Whittal, R. M.; Schriemer, D. C.; Li, L. Anal. Chem. 1997, 69, 2734–2741.

    Article  CAS  Google Scholar 

  18. Dogruel, D.; Nelson, R. W.; Williams, P. Rapid Commun. Mass Spectrom. 1996, 10, 801–804.

    Article  CAS  Google Scholar 

  19. Yates, H. T.; Scrivens, J.; Jackson, T.; Deery, M. In Proceedings of the 44th ASMS Conference on Mass Spectrometry and Allied Topics; Portland, OR, May 12–16, 1996; p 903.

  20. Jackson, A. T.; Yates, H. T.; MacDonald, W. A.; Scrivens, J. H.; Critchley, G.; Brown, J.; Deery, M. J.; Jennings, K. R.; Brookes, C. J. Am. Soc. Mass Spectrom. 1997, 8, 132–139.

    Article  CAS  Google Scholar 

  21. Cottrell, J. S.; Dwyer, J. L. In Proceedings of the 44th ASMS Conference on Mass Spectrometry and Allied Topics; Portland, OR, May 12–16, 1996; p 900.

  22. Kassis, C. M.; Belu, A. M.; DeSimone, J. M.; Linton, R. W.; Lange, G. W.; Friedman, R. M. In Proceedings of the 44th ASMS Conference on Mass Spectrometry and Allied Topics; Portland, OR, May 12–16, 1996; p 1096.

  23. Chen, H. R.; Guo, B. C. Anal. Chem. 1997, 69, 4399–4404.

    Article  CAS  Google Scholar 

  24. Dai, Y. Q.; Whittal, R. M.; Li, L. Anal. Chem. 1996, 68, 2494–2500.

    Article  CAS  Google Scholar 

  25. Whittal, R. M.; Li, L. Anal. Chem. 1995, 67, 1950–1954.

    Article  CAS  Google Scholar 

  26. Whittal, R. M.; Russon, L. M.; Weinberger, S. R.; Li, L. Anal. Chem. 1997, 69, 2147–2153.

    Article  CAS  Google Scholar 

  27. Schriemer, D. C.; Li, L. Anal. Chem. 1997, 69, 4176–4183.

    Article  CAS  Google Scholar 

  28. See, for example, Fuchs, O.; Suhr, H. H. In Polymer Handbook; Immergut, E. H.; McDowell, W., Eds.; Wiley: New York, 1975; pp IV241–333.

    Google Scholar 

  29. Schriemer, D. C.; Li, L. Anal. Chem. 1997, 69, 4169–4175.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Alberta, T6G 2G2, Edmonton, Alberta, Canada

    Talat Yalcin, Yuqin Dai & Liang Li

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  1. Talat Yalcin
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  2. Yuqin Dai
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  3. Liang Li
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Correspondence to Liang Li.

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Yalcin, T., Dai, Y. & Li, L. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for polymer analysis: solvent effect in sample preparation. J Am Soc Mass Spectrom 9, 1303–1310 (1998). https://doi.org/10.1016/S1044-0305(98)00109-3

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00109-3

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