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Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix

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Journal of the American Society for Mass Spectrometry

Abstract

Negative-ion matrix-assisted laser desorption/ionization mass spectra of sialylated glycoconjugates were acquired employing 2,5-dihydroxybenzoic acid (DHB) in conjunction with spermine as a co-matrix. The addition of spermine to DHB permitted an improved crystal formation as well as a higher analyte solubility. Moreover, DHB/spermine appears to minimize alkali adduct formation, thus allowing the sample analysis without desalting. The combined matrix permitted the analysis of complex sialylated and sialylated/fucosylated structures down to the femtomole range. The ability to use such a matrix also facilitates determination of the sialic acid linkages (in combination with a specific enzyme cleavage). The matrix also appears suitable for studies on gangliosides.

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Authors and Affiliations

  1. Department of Chemistry, Indiana University, Bloomington, Indiana, USA

    Yehia Mechref & Milos V. Novotny

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  1. Yehia Mechref
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  2. Milos V. Novotny
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Correspondence to Milos V. Novotny.

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Mechref, Y., Novotny, M.V. Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix. J Am Soc Mass Spectrom 9, 1293–1302 (1998). https://doi.org/10.1016/S1044-0305(98)00106-8

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00106-8

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