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Evidence for linkage position determination in cobalt coordinated pentasaccharides using ion trap mass spectrometry

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Journal of the American Society for Mass Spectrometry

Abstract

A methodology to determine the linkage position of oligosaccharides is presented. In order to illustrate this technique, several oligosaccharides and disaccharides were ionized by electrospray and analyzed in a Paul trap mass spectrometer. Multiple stage tandem mass spectrometry experiments were used to determine linkage and structural information for the following four cobalt coordinated and singly charged ([M+Co−H]+) pentasaccharides: Lacto-N-fucopentaose I, II, III, and V. In order to differentiate between linkage positions, multiple low energy collision induced experiments with mass selected C type ions have been carried out in an ion trap mass spectrometer. Because of the coordination with cobalt, which directs the dissociation pathways, these C type ions undergo specific fragmentation reactions upon low energy collision induced dissociation. These dissociation pathways are unambiguously dependent on their linkage position, thus allowing differentiation between 1→2, 1→3, 1→4, and 1→6 linkage positions throughout the oligomers. Studies on various linked disaccharides and N-acetyl-disaccharides, which are smaller constituents of the pentasaccharides, were used to verify and confirm the results obtained from the pentasaccharides.

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Authors and Affiliations

  1. College of Chemistry, University of California, Berkeley, California, USA

    Stefan König & Julie A. Leary

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  1. Stefan König
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  2. Julie A. Leary
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Correspondence to Julie A. Leary.

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König, S., Leary, J.A. Evidence for linkage position determination in cobalt coordinated pentasaccharides using ion trap mass spectrometry. J Am Soc Mass Spectrom 9, 1125–1134 (1998). https://doi.org/10.1016/S1044-0305(98)00096-8

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00096-8

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