Abstract
The product-ion spectra of the sodiated molecules of glycerol phosphatidylcholine phospholipids (GPC) were obtained, using fast-atom bombardment (FAB) as the ionization method, in a tandem mass spectrometer. The product-ion spectra of these sodiated molecules of GPCs were found to differ significantly from those of the protonated GPC molecules. This difference is due to the absence of the ion of m/z 184 (protonated-phosphocholine moiety) and to the presence of an ion resulting from the loss of trimethylamine (m=59 Da) from the polar head group, which is the dominant fragmentation. This characteristic neutral loss provides a means of identification of this class of phospholipids and of differentiation from other phospholipid classes in complex mixtures by performing a constant-neutral-loss scan of 59.
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References
Silvius, J. R. In Phospholipids Handbook; Gregor, Cevc, Ed.; Dekker: New York, 1993; pp 1–22.
Lehmann, W. D.; Kessler, M. Chem. Phys. Lipids 1983, 32, 123–135.
Heller, D. N.; Cotter, R. J.; Fenselau, C.; Uy, O. M. Anal. Chem. 1987, 59, 2806–2809.
Pramanik, B. N.; Zechman, J. M.; Das, P. R.; Bartner, P. L. Biomed. Environ. Mass Spectrom. 1990, 19, 164–170.
Cole, M. J.; Enke, C. G. Anal. Chem. 1991, 63, 1032–1038.
Bryant, D. K.; Orlando, R. C.; Fenselau, C.; Sowder, R. C.; Henderson, L. E. Anal. Chem. 1991, 63, 1110–1114.
Murphy, R. C. In Handbook of Lipid Research, Vol. 7; Plenum: New York, 1993; Chap 7, pp 213–252.
Clay, K. L.; Wahlin, L.; Murphy, R. C. Biomed Mass Spectrom 1983, 10, 489–494.
Jensen, N. J.; Tomer, K. B.; Gross, M. L. Lipids 1986, 21, 580–588.
Easton, C.; Johnson, D. W.; Poulos, A. J. Lipid Res. 1988, 29, 109–112.
Han, X.; Gross, R. W. J. Am. Soc Mass Spectrom. 1995, 6, 1202–1210.
Han, X.; Gross, R. W. J. Am. Chem. Soc. 1996, 118, 451–457.
Heller, D. N.; Murphy, C. M.; Cotter, R. J.; Fenselau, C.; Uy, O. M. Anal. Chem. 1988, 60, 2787–2791.
This procedure has been utilized during the revision phase of this manuscript by suggestion of the reviewers.
Crawford, C. G.; Plattner, R. D. J. Lipid Res. 1983, 24, 456–460.
Weintraub, S. T.; Pinckard, R. N.; Hail, M. Rapid Commun. Mass Spectrom. 1991, 5, 309–311.
Zirrolli, J. A.; Clay, K. L.; Murphy, R. L. Lipids 1991, 23, 1112–1116.
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Domingues, P., Amado, F.M.L., Santana-Marques, M.G.O. et al. Constant neutral loss scanning for the characterization of glycerol phosphatidylcholine phospholipids. J Am Soc Mass Spectrom 9, 1189–1195 (1998). https://doi.org/10.1016/S1044-0305(98)00087-7
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DOI: https://doi.org/10.1016/S1044-0305(98)00087-7