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Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange

  • Focus: Maldi
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Journal of the American Society for Mass Spectrometry

Abstract

The fragmentation of positive ions of DNA under the conditions of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was investigated by post-source decay (PSD) analysis and hydrogen/deuterium (H/D) exchange. Spectra of five different synthetic 4mer oligonucleotides were recorded. As a main result the hypothesis was confirmed that for these ions all fragment ions result from processes, initiated by protonation/deuteration of a suitable base followed by a loss of this base as a neutral or ion and further backbone cleavages. The three bases adenine, guanine, and cytosine all exhibit comparable lability for fragmentation. The spectra show evidence for an interaction of the adenine base with the phosphate backbone. Signals of fragments containing TT- and CT-cycloadducts were observed in the spectra.

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Authors and Affiliations

  1. Institute for Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, D-48149, Münster, Germany

    Julia Gross, Arne Leisner & Franz Hillenkamp

  2. Zentrum für Umweltforschung und Technologie, Genom Analytik GmbH, Bremen, Germany

    Stephanie Hahner

  3. Abteilung für Instrumentelle Analytik, Universitaet Frankfurt, Frankfurt/Main, Germany

    Michael Karas & Jürgen Schäfer

  4. Institut fuer Lasermedizin, Universitaet Duesseldorf, Duesseldorf, Germany

    Frank Lützenkirchen

  5. Institute for Molecular Biology, Odense University, Odense M, Denmark

    Eckhard Nordhoff

Authors
  1. Julia Gross
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  2. Arne Leisner
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  3. Franz Hillenkamp
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  4. Stephanie Hahner
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  5. Michael Karas
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  6. Jürgen Schäfer
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  7. Frank Lützenkirchen
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  8. Eckhard Nordhoff
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Correspondence to Franz Hillenkamp.

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Gross, J., Leisner, A., Hillenkamp, F. et al. Investigations of the metastable decay of DNA under ultraviolet matrix-assisted laser desorption/ionization conditions with post-source-decay analysis and hydrogen/deuterium exchange. J Am Soc Mass Spectrom 9, 866–878 (1998). https://doi.org/10.1016/S1044-0305(98)00058-0

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00058-0

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