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Origin of product ions in the MS/MS spectra of peptides in a quadrupole ion trap

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Journal of the American Society for Mass Spectrometry

Abstract

Stored waveform inverse Fourier transform and double resonance techniques have been used in conjunction with a quadrupole ion trap to study the dissociation patterns of peptide ions. These experiments provide insight into the origin of individual product ions in an MS/MS spectrum. Results show for a series of leucine enkephalin analogues with five amino acid residues that the b 4 ion is the main product ion through which many other product ions arise. It was also observed that the percentage of the a 4 product ions that are formed directly from the protonated molecule (M+H)+ depends on the nature of the fourth amino acid residue. In addition, it was determined that in the peptides studied here lower series b ions (e.g., b 3 arise from direct dissociation of higher series b ions (e.g., b 4 only about 50% of the time.

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Authors and Affiliations

  1. Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA

    Richard W. Vachet, Kenneth L. Ray & Gary L. Glish

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  1. Richard W. Vachet
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  2. Kenneth L. Ray
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  3. Gary L. Glish
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Correspondence to Gary L. Glish.

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Vachet, R.W., Ray, K.L. & Glish, G.L. Origin of product ions in the MS/MS spectra of peptides in a quadrupole ion trap. J Am Soc Mass Spectrom 9, 341–344 (1998). https://doi.org/10.1016/S1044-0305(98)00008-7

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  • DOI: https://doi.org/10.1016/S1044-0305(98)00008-7

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