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Simultaneous analysis of 14 non-steroidal anti-inflammatory drugs in human serum by electrospray ionization-tandem mass spectrometry without chromatography

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Journal of the American Society for Mass Spectrometry

Abstract

A method is described for the simultaneous analysis of 14 non-steroidal anti-inflammatory drugs (NSAIDS) in human serum using negative electrospray ionization-tandem mass spectrometry (ESI-MS/MS). After addition of internal standard and protein precipitation using acetonitrile, samples were transferred to autosampler vials for direct analysis without chromatography. Injection of an air bubble with the sample and a multiple reaction monitoring (MRM) method using argon collision-induced dissociation (CID) of analyte (M-H)− ions permitted integration of the product ion peak areas to produce reproducible quantitative data over the range of concentrations expected in serum during routine use of these drugs. The method permitted the analysis of 30 samples per hour. Two hundred fifty consecutive analyses did not adversely affect instrument sensitivity.

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References

  1. Baeyens, W. R. G.; Van der Weken, G.; Lievens, L.; Van Overbeke, A. Biomed. Chromatogr. 1995, 9, 263–264.

    Article  CAS  Google Scholar 

  2. Baeyens, W. R. G.; Van der Weken, G.; Van Overbeke, A.; Shang, Z. D. Biomed. Chromatogr. 1995, 9, 261–262.

    Article  CAS  Google Scholar 

  3. Giachetti, C.; Zanolo, G.; Poletti, P.; Perovanni, F. J. High Resolut. Chromatogr. 1990, 13, 789–792.

    Article  CAS  Google Scholar 

  4. Kim, K.-R.; Yoon, H.-R. J. Chromatogr. B 1996, 682, 55–66.

    Article  CAS  Google Scholar 

  5. Singh, A. K.; Jang, Y.; Mishra, U.; Granley, K. J. Chromatogr. B Biomed. Appl. 1991, 106, 351–361.

    Google Scholar 

  6. Borensteink, M. R.; Xue, Y.; Cooper, S.; Tzeng, T.-B. J. Chromatogr. B Biomed. Appl. 1996, 685, 59–66.

    Article  Google Scholar 

  7. Gonzales, G.; Ventura, R.; Smith, A. K.; de la Tore, R.; Segura, J. J. Chromatogr. A 1996, 719, 251–264.

    Article  Google Scholar 

  8. Ashcroft, A. E.; Major, H. J.; Lowes, S.; Wilson, I. D. Anal. Proc. 1995, 32, 459–462.

    CAS  Google Scholar 

  9. Stanely, S. M. R.; Owens, N. A.; Rodgers, J. P. J. Chromatogr. B Biomed. Appl. 1995, 667, 95–103.

    Article  Google Scholar 

  10. Hambleton, P.; Lough, W. J.; Maltas, J.; Mills, M. J. J. Liq. Chromatogr. 1995, 18, 3205–3217.

    Article  CAS  Google Scholar 

  11. Montoya-Iraheta, C.; Garg, D. C.; Jallad, N. S.; Mroszzak, E. S.; Yee, J. P.; Renneke, G. A.; Weidler, D. J. J. Clin. Pharm. 1986, 26, 545.

    Google Scholar 

  12. Miehlke, R. K.; Schneider, S.; Sörgel, F.; Muth, P.; Henschke, F.; Giersch, K. H.; Münzel, P. Drugs 1990, 40, 57–61.

    Article  Google Scholar 

  13. Grindel, J. M.; Migdalof, B. H.; Plostinieks, J. Clin. Pharmacol. Ther. 1979, 2, 122–128.

    Google Scholar 

  14. Richardson, C. J.; Blocks, K. L. N.; Ross, S. G.; Verbeeck, R. K. Clin. Pharmacol. Ther. 1985, 37, 13–18.

    Article  CAS  Google Scholar 

  15. Weber, S. S.; Bankhurst, A. D.; Mroszczak, E.; Ding, T. L. Ther. Drug Monit. 1981, 3, 75–83.

    CAS  Google Scholar 

  16. Tomson, G.; Lunell, N. O.; Oliw, E.; Rane, A. Clin. Pharmacol. Ther. 1981, 29, 168–173.

    Article  CAS  Google Scholar 

  17. Upton, R. A.; Williams, R. L.; Guentert, T. W.; Buskin, J. N.; Riegelman, S. Eur. J. Clin. Pharmacol. 1981, 20, 127–133.

    Article  CAS  Google Scholar 

  18. Advenier, C.; Roux, A.; Gobert, C. Br. J. Clin. Pharmacol. 1983, 16, 65–70.

    CAS  Google Scholar 

  19. Albert, K. S.; Gillespie, W. R.; Raabe, A.; Garry, M. J. Pharmacol. Sci. 1984, 73, 1823–1825.

    Article  CAS  Google Scholar 

  20. Collier, P. S.; D’Arey, P. F.; Harron, D. W. G.; Morrow, N. Br. J. Clin. Pharmacol. 1978, 5, 528–530.

    CAS  Google Scholar 

  21. Vangiessen, G. J.; Kaiser, D. G. J. Pharm. Sci. 1975, 64, 798–801

    Article  CAS  Google Scholar 

  22. Ferdinandi, E.; Schgal, S. N.; Demerson, C. A. Xenobiotica 1986, 16, 153–166.

    Article  CAS  Google Scholar 

  23. Kraml, M.; Cosyns, L.; Hicks, D. R.; et al. Biopharm. Drug Dispos. 1984, 5, 63–74.

    Article  CAS  Google Scholar 

  24. Willis, J. V.; Kendall, M. J.; Jack, D. B. Eur. J. Clin. Pharm. 1981, 19, 33–37.

    Article  CAS  Google Scholar 

  25. Settlage, V. J. A.; Gielsdorf, W.; Nieder, M. et al. Arzneim. Forsch. Drug Res. 1983, 33, 885–888.

    CAS  Google Scholar 

  26. Glazko, A. J. Ann. Phys. Med. 1968, 9, Suppl. 23–36.

    Google Scholar 

  27. Rubin, A.; Rodda, B. E.; Warrick, P. et al. J. Pharmacol. Sci. 1971, 60, 1797–1801.

    Article  CAS  Google Scholar 

  28. Daypro® Package Insert, G. D. Searle and Co. Skokie, IL, Feb. 20, 1997.

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Authors and Affiliations

  1. National Medical Services, Inc., Willow Grove, Pennsylvania, USA

    John de Kanel, William E. Vickery & Francis X. Diamond

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  1. John de Kanel
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  2. William E. Vickery
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  3. Francis X. Diamond
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de Kanel, J., Vickery, W.E. & Diamond, F.X. Simultaneous analysis of 14 non-steroidal anti-inflammatory drugs in human serum by electrospray ionization-tandem mass spectrometry without chromatography. J Am Soc Mass Spectrom 9, 255–257 (1998). https://doi.org/10.1016/S1044-0305(97)00289-4

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  • DOI: https://doi.org/10.1016/S1044-0305(97)00289-4

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