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A mechanism for the loss of 60 u from peptides containing an arginine residue at the C-terminus

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Journal of the American Society for Mass Spectrometry

Abstract

The loss of 60 u from protonated peptide ions containing an arginine residue at the C-terminus has been investigated by means of low energy tandem mass spectrometry. The lowest energy conformation of singly charged bradykinin is thought to involve a salt-bridge structure, which may lead to the formation of two isomeric forms. It is thought that one isomer retains the ionizing proton at the C-terminal end of the peptide, leading to the formation of the [b n−1 + H + OH]+ fragment ion, and the other isomer retains the charge at the N-terminus, leading to the formation of the [M + H − 60]+ fragment ion. It was found that the formation of the [M + H − 60]+ ion occurs only from singly charged precursor ions. In addition, the loss of 60 u occurs from peptides in which the charge is localized at the N-terminus. These results indicate that the mechanism of formation of the [M + H − 60]+ ion may be driven by a charge-remote process.

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Authors and Affiliations

  1. Institute of Mass Spectrometry, Department of Chemistry, University of Warwick, Coventry, UK

    Michael J. Deery, Scott G. Summerfield, Armelle Buzy & Keith R. Jennings

Authors
  1. Michael J. Deery
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  2. Scott G. Summerfield
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  3. Armelle Buzy
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  4. Keith R. Jennings
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Deery, M.J., Summerfield, S.G., Buzy, A. et al. A mechanism for the loss of 60 u from peptides containing an arginine residue at the C-terminus. J Am Soc Mass Spectrom 8, 253–261 (1997). https://doi.org/10.1016/S1044-0305(96)00197-3

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  • DOI: https://doi.org/10.1016/S1044-0305(96)00197-3

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