Abstract
A new atmospheric pressure (AP) infrared (IR) matrix-assisted laser desorption/ionization (MALDI) ion source was developed and interfaced with a Thermo Finnigan LCQ ion trap mass spectrometer. The source utilized a miniature all-solid-state optical parametric oscillator (OPO)-based IR laser system tunable in the λ = 1.5–4 μm spectral range and a nitrogen ultraviolet (UV) laser (λ = 337 nm) for use in comparative studies. The system demonstrated comparable performance at 3 μm and 337 ran wavelengths if UV matrices were used. However, AP IR-MALDI using a 3 μm wavelength showed good performance with a much broader choice of matrices including glycerol and liquid water. AP IR-MALDI mass spectra of peptides in the mass range up to 2000 Da were obtained directly from aqueous solutions at atmospheric conditions for the first time. A potential use of the new AP IR-MALDI ion source includes direct MS analysis of biological cells and tissues in a normal atmospheric environment as well as on-line coupling of mass spectrometers with liquid separation techniques.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Overberg, A.; Karas, M.; Bahr, U.; Kaufmann, R.; Hillenkamp, F. Rapid Commun. Mass Spectrom. 1990, 4, 293–296.
Karas, M.; Hillenkamp, F. Anal. Chem. 1988, 60, 2299–2301.
Tanaka, K.; Ido, Y.; Akita, S.; Yoshida, Y.; Yoshida, T. Rapid Commun. Mass Spectrom. 1988, 2, 151–153.
Nordhoff, E.; Ingendoh, A.; Cramer, R.; Overberg, A.; Stahl, B.; Karas, M.; Hillenkamp, F.; Crain, P. F. Rapid Commun. Mass Spectrom. 1992, 6, 771–776.
Berkenkamp, S.; Menzel, C.; Karas, M.; Hillenkamp, F. Rapid Commun. Mass Spectrom. 1997, 11, 1399–1406.
Niu, S.; Zhang, W.; Chait, B. T. J. Am. Soc. Mass Spectrom. 1998, 9, 1–7.
Berkenkamp, S.; Kirpekar, F.; Hillenkamp, F. Science 1998, 281, 260–262.
Schneider, K.; Chait, B. T. Org. Mass Spectrom. 1993, 28, 1353–1361.
Fitzgerald, M. C.; Parr, G. R.; Smith, L. M. Anal. Chem. 1993, 65, 3204–3211.
Talrose, V. L.; Person, M. D.; Whittal, R. M.; Walls, F. C.; Burlingame, A. L.; Baldwin, M. A. Rapid Commun. Mass Spectrom. 1999, 13, 2191–2198.
Ryzhov, V.; Bundy, J. L.; Fenselau, C.; Taranenko, N.; Doroshenko, V.; Prasad, C. R. Rapid Commun. Mass Spectrom. 2000, 14, 1701–1706.
Berkenkamp, S.; Karas, M.; Hillenkamp, F. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 7003–7007.
Cramer, R.; Hillenkamp, F.; Haglung, R. F. J. Am. Soc. Mass Spectrom. 1996, 7, 1187–1193.
Nelson, R. W.; Thomas, R. M.; Williams, P. Rapid Commun. Mass Spectrom. 1990, 4, 348–351.
Schieltz, D. M.; Chou, C.-W.; Luo, C.-W.; Thomas, R. M.; Williams, P. Rapid Commun. Mass Spectrom. 1992, 6, 631–636.
Hunter, J. M.; Lin, H.; Becker, C. H. Anal. Chem. 1997, 69, 3608–3612.
Kraft, P.; Alimpiev, S.; Dratz, E.; Sunner, J. J. Am. Soc. Mass Spectrom. 1998, 9, 912–924.
Caldwell, K. L.; Murray, K. K. Appl. Surf. Sci. 1998, 127/129, 242–247.
Sheffer, J. D.; Murrey, K. K. J. Mass Spectrom. 2000, 35, 95–97.
Ullom, J. N.; Frank, M.; Gard, E. E.; Horn, J. M.; Labov, S. E.; Langry, K.; Magnotta, F.; Stanion, K. A.; Hack, C. A.; Benner, W. H. Anal. Chem. 2001, 73, 2331–2337.
Wattenberg, A.; Sobott, F.; Barth, H.-D.; Brutschy, B. Eur. J. Mass Spectrom. 1999, 5, 71–76.
Wattenberg, A.; Sobott, F.; Brutschy, B. Rapid Commun. Mass Spectrom. 2000, 14, 859–861.
Laiko, V. V.; Baldwin, M. A.; Burlingame, A. L. Anal. Chem. 2000, 72, 652–657.
Laiko, V. V.; Moyer, S. C.; Cotter, R. J. Anal. Chem. 2000, 72, 5239–5243.
Lin, B.; Sunner, J. J. Am. Soc. Mass Spectrom. 1994, 5, 873–885.
Callahan, J. H.; Galicia, M.; Vertes, A. Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics; Long Beach, CA, June, 2000.
Danell, R. M.; Glish, G. L. Proceedings of the 48th ASMS Conference on Mass Spectrometry and Allied Topics; Long Beach, CA, June, 2000.
Doroshenko, V. M.; Cornish, T. J.; Cotter, R. J. Rapid Commun. Mass Spectrom. 1992, 6, 753–757.
Doroshenko, V. M.; Cotter, R. J. Rapid Commun. Mass Spectrom. 1993, 7, 822–827.
Doroshenko, V. M.; Cotter, R. J. J. Mass Spectrom. 1997, 31, 602–615.
Hiraoka, K.; Saito, S.; Katsuragawa, J.; Kudaka, I. Rapid Commun. Mass Spectrom. 1998, 12, 1170–1174.
Kudaka, I.; Kojima, T.; Saito, S.; Hiraoka, K. Rapid Commun. Mass Spectrom. 2000, 14, 1558–1562.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Laiko, V.V., Taranenko, N.I., Berkout, V.D. et al. Desorption/ionization of biomolecules from aqueous solutions at atmospheric pressure using an infrared laser at 3 μm. J Am Soc Mass Spectrom 13, 354–361 (2002). https://doi.org/10.1016/S1044-0305(02)00341-0
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/S1044-0305(02)00341-0