Abstract
Data-dependent external m/z selection and accumulation of ions is demonstrated in use with ESI-FTICR instrumentation, with two different methods for ion selection being explored. One method uses RF/DC quadrupole filtering and is described in use with an 11.5 tesla (T) FTICR instrument, while the second method employs RF-only resonance dipolar excitation selection and is described in use with a 3.5 T FTICR instrument. In both methods ions are data-dependently selected on the fly in a linear quadrupole ion guide, then accumulated in a second linear RF-only quadrupole trap that immediately follows. A major benefit of ion preselection prior to external accumulation is the enhancement of ion populations for low-level species. This development is expected to expand the dynamic range and sensitivity of FTICR for applications including analysis of complex polypeptide mixtures (e.g., proteomics).
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Harkewicz, R., Belov, M.E., Anderson, G.A. et al. ESI-FTICR mass spectrometry employing Data-dependent external ion selection and accumulation. J Am Soc Mass Spectrom 13, 144–154 (2002). https://doi.org/10.1016/S1044-0305(01)00343-9
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DOI: https://doi.org/10.1016/S1044-0305(01)00343-9