Skip to main content
SpringerLink
Log in

Microfabricated PDMS multichannel emitter for electrospray ionization mass spectrometry

  • Published:
Journal of the American Society for Mass Spectrometry

Abstract

A novel microfabricated multichannel emitter for electrospray ionization mass spectrometry (ESI-MS) was implemented with polydimethylsiloxane (PDMS) using a soft lithography technique. The emitters are formed as electrospray tips along a thin membrane on the edge of the device with channels of 100 μm × 30 μm dimensions. The electrospray performance of the PDMS emitters for a single channel device and a four channel device interfaced with a time-of-flight mass spectrometer was evaluated for detecting the molecular weight of reference peptides (angiotensin I and bradykinin). The emitters were durable at the flow rate of 1–20 μL min−1 for more than 30 h of continuous electrospray with limit of detection of 1 μM (S/N 18). This microfabrication method for a PDMS multichannel emitter as an integral part of a microfluidic device will facilitate development of more complex microfluidic analysis systems using ESI-MS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ramsey, J. M.; Jacobson, S. C.; Knapp, M. R. Nat. Med. 1995, 1, 1093–1096.

    Article  CAS  Google Scholar 

  2. Regnier, F. E.; He, B.; Lin, S.; Busse, J. Trends Biotechnol, 1999, 17, 101–106.

    Article  CAS  Google Scholar 

  3. Oleschuk, R. D.; Harrison, D. J. TrAC, Trends Anal. Chem. 2000, 19, 379–388.

    Article  CAS  Google Scholar 

  4. Jakeway, S. C.; de Mello, A. J.; Russell, E. L. Fresenius’ J. Anal. Chem. 2000, 366, 525–539.

    Article  CAS  Google Scholar 

  5. Xue, Q.; Foret, F.; Dunayevskiy, Y. M.; Zavracky, P. M.; McGruer, N. E.; Karger, B. L. Anal. Chem. 1997, 69, 426–430.

    Article  CAS  Google Scholar 

  6. Ramsey, R. S.; Ramsey, J. M. Anal. Chem. 1997, 69, 1174–1178.

    Article  CAS  Google Scholar 

  7. Figeys, D.; Ning, Y.; Aebersold, R. Anal. Chem. 1997, 69, 3153–3160.

    Article  CAS  Google Scholar 

  8. Figeys, D.; Aebersold, R. Anal. Chem. 1998, 70, 3721–3727.

    Article  CAS  Google Scholar 

  9. Figeys, D.; Gygi, S. P.; McKinnon, G.; Aebersold, R. Anal. Chem. 1998, 70, 3728–3734.

    Article  CAS  Google Scholar 

  10. Zhang, B.; Liu, H.; Karger, B. L.; Foret, F. Anal. Chem. 1999, 71, 3258–3264.

    Article  CAS  Google Scholar 

  11. Lazar, I. M.; Ramsey, R. S.; Sundberg, S.; Ramsey, J. M. Anal. Chem. 1999, 71, 3627–3631.

    Article  CAS  Google Scholar 

  12. Li, J.; Thibault, P.; Bings, N. H.; Skinner, C. D.; Wang, C.; Colyer, C.; Harrison, D. J. Anal. Chem. 1999, 71, 3036–3045.

    Article  CAS  Google Scholar 

  13. Chan, J. H.; Timperman, A. T.; Qin, D.; Aebersold, R. Anal. Chem. 1999, 71, 4437–4444.

    Article  CAS  Google Scholar 

  14. Zhang, B.; Foret, F.; Karger, B. L. Anal. Chem. 2000, 72, 1015–1022.

    Article  CAS  Google Scholar 

  15. Pinto, D. M.; Ning, Y. B.; Figeys, D. Electrophoresis 2000, 21, 181–190.

    Article  CAS  Google Scholar 

  16. Liu, H.; Felten, C.; Xue, Q.; Zhang, B.; Jodrzejewski, P.; Karger, B. L.; Foret, F. Anal. Chem. 2000, 72, 3303–3310.

    Article  CAS  Google Scholar 

  17. Desai, A.; Tai, Y.-C.; Davis, M. T.; Lee, T. D. A MEMS electrospray nozzle for mass spectroscopy. 1997 International conference on solid state sensors and actuators (Transducers ’97), May, 1997, Piscataway, NJ; 927–930.

  18. Licklider, L.; Wang, X. Q.; Desai, A.; Tai, Y. C.; Lee, T. D. Anal. Chem. 2000, 21, 367–375.

    Article  CAS  Google Scholar 

  19. Wen, J.; Lin, Y.; Xiang, F.; Matson, D. W.; Udseth, H. R.; Smith, R. D. Electrophoresis 2000, 21, 191–198.

    Article  CAS  Google Scholar 

  20. Schultz, G. A.; Corso, T. N.; Prosser, S. J.; Zhang, S. Anal. Chem. 2000, 72, 4058–4063.

    Article  CAS  Google Scholar 

  21. Xia, Y.; Whitesides, G. M. Angew. Chem. Int. Ed. 1998, 37, 551–575.

    Article  Google Scholar 

  22. Qin, D.; Xia, Y.; Whitesides, G. M. Adv. Mater. 1996, 8, 917–919.

    Article  CAS  Google Scholar 

  23. Duffy, D. C.; McDonald, J. C.; Schueller, O. J. A.; Whitesides, G. M. Anal. Chem. 1998, 70, 4974–4984.

    Article  CAS  Google Scholar 

  24. Anderson, J. R.; Chiu, D. T.; Jackman, R. J.; Cherniavskaya, O.; McDonald, J. C.; Wu, H.; Whitesides, S. H.; Whitesides, G. M. Anal Chem. 2000, 72, 3158–3164.

    Article  CAS  Google Scholar 

  25. McDonald, J. C.; Duffy, D. C.; Anderson, J. R.; Chiu, D. T.; Wu, H. K.; Schueller, O. J. A.; Whitesides, G. M. Electrophoresis 2000, 21, 27–40.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Medical University of South Carolina, Charleston, South Carolina, USA

    Jin-Sung Kim & Daniel R. Knapp

Authors
  1. Jin-Sung Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daniel R. Knapp
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Daniel R. Knapp.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, JS., Knapp, D.R. Microfabricated PDMS multichannel emitter for electrospray ionization mass spectrometry. J Am Soc Mass Spectrom 12, 463–469 (2001). https://doi.org/10.1016/S1044-0305(01)00219-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1044-0305(01)00219-7

Keywords

Search

Navigation