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5-Methoxysalicylic acid and spermine: A new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides

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Journal of the American Society for Mass Spectrometry

Abstract

5-Methoxysalicylic acid (MSA) is demonstrated to be a useful matrix for matrix-assisted laser desorption/ionization time-of-flight (TOF) mass spectrometry of oligonucleotides, when desorption/ionization without fragmentation is desired. When MSA is combined with the additive spermine, the need for desalting is reduced. The MSA/spermine matrix yields linear TOF mass spectra with improved resolution, less fragmentation, and less intense alkali ion adduct peaks than those spectra obtained using 3-hydroxypicolinic acid and 6-aza-2-thiothymine with spermine or diammonium hydrogen citrate as additives. Instrumental conditions are discussed to improve the spectral resolution, specifically the use of longer delay times in the delayed-extraction ion source.

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Authors and Affiliations

  1. Department of Chemistry, Michigan State University, East Lansing, Michigan, USA

    Anne M. Distler & John Allison

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  1. Anne M. Distler
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  2. John Allison
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Correspondence to John Allison.

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Distler, A.M., Allison, J. 5-Methoxysalicylic acid and spermine: A new matrix for the matrix-assisted laser desorption/ionization mass spectrometry analysis of oligonucleotides. J Am Soc Mass Spectrom 12, 456–462 (2001). https://doi.org/10.1016/S1044-0305(01)00212-4

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  • DOI: https://doi.org/10.1016/S1044-0305(01)00212-4

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