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Mechanistic investigation of ionization suppression in electrospray ionization

  • Focus: Electrospray
  • Published:
Journal of the American Society for Mass Spectrometry

Abstract

We show results from experiments designed to determine the relative importance of gas phase processes and solution phase processes into ionization suppression observed in biological sample extracts. The data indicate that gas phase reactions leading to the loss of net charge on the analyte is not likely to be the most important process involved in ionization suppression. The results point to changes in the droplet solution properties caused by the presence of nonvolatile solutes as the main cause of ionization suppression in electrospray ionization of biological extracts.

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Authors and Affiliations

  1. Department of Drug Metabolism, Merck Research Laboratories, West Point, Pennsylvania, USA

    Richard King, Ryan Bonfiglio, Carmen Fernandez-Metzler, Cynthia Miller-Stein & Timothy Olah

Authors
  1. Richard King
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  2. Ryan Bonfiglio
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  3. Carmen Fernandez-Metzler
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  4. Cynthia Miller-Stein
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  5. Timothy Olah
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Correspondence to Richard King.

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King, R., Bonfiglio, R., Fernandez-Metzler, C. et al. Mechanistic investigation of ionization suppression in electrospray ionization. J. Am. Soc. Spectrom. 11, 942–950 (2000). https://doi.org/10.1016/S1044-0305(00)00163-X

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  • DOI: https://doi.org/10.1016/S1044-0305(00)00163-X

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