Skip to main content
SpringerLink
Log in

Endgroup determination of synthetic polymers by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

  • Published:
Journal of the American Society for Mass Spectrometry

Abstract

Electrospray ionization (ESI) was performed on a Fourier transform ion cyclotron resonance mass spectrometer for the endgroup and monomer mass determination of three poly(oxyalkylene)s in the mass range of 400–8000 Da. A combined use of the multiple charge states observed with ESI, leads to a threefold increase in accuracy of the endgroup and monomer determination. The improvement is attributed to the increased number of datapoints used for the regression procedure, yielding more accurate results. Endgroup masses are determined with a mass error better than 5 and 75 millimass units for the molecular weight range of 400–4200 and 6200–8000 Da, respectively. A mass error of better than 1 millimass unit was observed for all monomer mass determinations. With ESI, endgroup and monomer masses have been determined for poly(ethylene glycol) oligomers with a mass higher than 8000 Da. This is almost two times higher than observed with matrix-assisted laser desorption/ ionization on the same instrument.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Montaudo, G.; Montaudo, M. S.; Puglisi, C.; Samperi, F. Rapid Commun. Mass Spectrom. 1995, 9, 453–460.

    Article  CAS  Google Scholar 

  2. Montaudo, G.; Garozzo, D.; Montaudo, M. S.; Puglisi, C.; Samperi, F. Macromolecules 1995, 28, 7983–7989.

    Article  CAS  Google Scholar 

  3. Nielen, M. W. F.; Malucha, S. Rapid Commun. Mass Spectrom. 1997, 11, 1194–1204.

    Article  CAS  Google Scholar 

  4. Easterling, M. L.; Mize, T. H.; Amster, I. J. Int. J. Mass Spectrom. Ion Processes 1997, 169/170, 387–400.

    Article  CAS  Google Scholar 

  5. McLafferty, F. W. Acc. Chem. Res. 1994, 27, 379–386.

    Article  CAS  Google Scholar 

  6. van Rooij, G. J.; Duursma, M. C.; Heeren, R. M. A.; Boon, J. J.; de Koster, C. G. J. Am. Soc. Mass Spectrom. 1996, 7, 449–457.

    Article  Google Scholar 

  7. Alber, G. M.; Marshall, A. G.; Hill, N. C.; Schweikhard, L.; Ricca, T. Rev. Sci. Instrum. 1993, 64, 1845–1852.

    Article  CAS  Google Scholar 

  8. Dey, M.; Castoro, J. A.; Wilkins, C. L. Anal. Chem. 1995, 67, 1575–1579.

    Article  CAS  Google Scholar 

  9. O’Connor, P. B.; Duursma, M. C.; van Rooij, G. J.; Heeren, R. M. A.; Boon, J. J. Anal. Chem. 1997, 69, 2751–2755.

    Article  Google Scholar 

  10. Prokai, L.; Simonsick, W. J., Jr. Rapid Commun. Mass Spectrom. 1993, 7, 853–856.

    Article  CAS  Google Scholar 

  11. Nielen, M. W. F. Rapid Commun. Mass Spectrom. 1996, 10, 1652–1660.

    Article  CAS  Google Scholar 

  12. Reinhold, M.; Meier, R. J.; de Koster, C. G. Rapid Commun. Mass Spectrom. 1998, 12, 1962–1966.

    Article  CAS  Google Scholar 

  13. Wong, S. F.; Meng, C. K.; Fenn, J. B. J. Phys. Chem. 1988, 92, 546–550.

    Article  CAS  Google Scholar 

  14. Jasieczek, C. B.; Buzy, A.; Haddleton, D. M.; Jennings, K. R. Rapid Commun. Mass Spectrom. 1996, 10, 509–514.

    Article  CAS  Google Scholar 

  15. McEwen, C. N.; Simonsick, W. J., Jr.; Larsen, B. S.; Ute, K.; Hatada, K. J. Am. Soc. Mass Spectrom. 1995, 6, 906–911.

    Article  CAS  Google Scholar 

  16. Hofstadler, S. A.; Beu, S. C.; Laude, D. A., Jr. Anal. Chem. 1993, 65, 312–316.

    Article  CAS  Google Scholar 

  17. Gard, E. E.; Green, M. K.; Warren, H.; Camara, E. J. O.; He, F.; Penn, S. G.; Lebrilla, C. B. Int. J. Mass Spectrom. Ion Processes 1996, 157/158, 115–127.

    Article  CAS  Google Scholar 

  18. O’Connor, P. B.; McLafferty, F. W. J. Am. Chem. Soc. 1995, 117, 12826–12831.

    Article  Google Scholar 

  19. Schmidt, E. G.; Karpinski, M. L.; Bolanos, B. J.; Harper, C. J.; Weil, D. A.; Laude, D. A. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics; Palm Springs, 1997; p 881.

  20. Mize, T. H.; Pitsenberger, C. C.; Easterling, M. L.; Danis, P.; Amster, I. J. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics; Palm Springs, 1997; p 542.

  21. Pastor, S. J.; Wilkins, C. L. Proceedings of the 45th ASMS Conference on Mass Spectrometry and Allied Topics; Palm Springs, 1997; p 537.

  22. van der Hage, E. R. E.; Duursma, M. C.; Heeren, R. M. A.; Boon, J. J.; Nielen, M. W. F.; Weber, A. J. M.; de Koster, C. G.; de Vries, N. K. Macromolecules 1997, 30, 4302–4309.

    Article  Google Scholar 

  23. de Koster, C. G.; Duursma, M. C.; van Rooij, G. J.; Heeren, R. M. A.; Boon, J. J. Rapid Commun. Mass Spectrom. 1995, 9, 957–962.

    Article  Google Scholar 

  24. Easterling, M. L.; Amster, I. J.; van Rooij, G. J.; Heeren, R. M. A. J. Am. Soc. Mass Spectrom., submitted.

  25. Caravatti, P.; Allemann, M. Org. Mass Spectrom. 1991, 26, 514–518.

    Article  CAS  Google Scholar 

  26. Heeren, R. M. A.; de Koster, C. G.; Boon, J. J. Anal. Chem. 1995, 67, 3965–3970.

    Article  CAS  Google Scholar 

  27. Heeren, R. M. A.; Boon, J. J. Int. J. Mass Spectrom. Ion Processes 1996, 157/158, 391–403.

    Article  CAS  Google Scholar 

  28. Nohmi, T.; Fenn, J. B. J. Am. Chem. Soc. 1992, 114, 3241–3246.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Unit for Macromolecular Mass Spectrometry, FOM-Institute for Atomic and Molecular Physics, Amsterdam, The Netherlands

    Sander Koster, Marc C. Duursma, Jaap J. Boon & Ron M. A. Heeren

Authors
  1. Sander Koster
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marc C. Duursma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaap J. Boon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ron M. A. Heeren
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ron M. A. Heeren.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koster, S., Duursma, M.C., Boon, J.J. et al. Endgroup determination of synthetic polymers by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom 11, 536–543 (2000). https://doi.org/10.1016/S1044-0305(00)00115-X

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1044-0305(00)00115-X

Keywords

Search

Navigation