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Accurate mass measurement of low molecular weight compounds by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Journal of the American Society for Mass Spectrometry

Abstract

We report the application of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the accurate measurement of mass of low molecular weight compounds (smaller than 1500 Da), a linear peptide, two types of cyclic depsipeptides, a polyhydroxy-macrocyclic lactone, and two prenylated flavonoids, with delayed extraction in the reflector mode. The performance of the MALDI-TOF instrument was less than those of fast atom bombardment and Fourier-transform ion cyclotron resonance mass spectrometry instruments and insufficient to give acceptable accuracy for literature reporting. Nevertheless, when combined with NMR spectrometry and/or amino acid analysis to give information on the numbers of carbon atoms and index of hydrogen deficiency, MALDI was useful for determination of the elemental composition of the low molecular weight compounds available in small quantities.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi, 274-8510, Chiba, Japan

    Toshio Fukai, Jun Kuroda & Taro Nomura

Authors
  1. Toshio Fukai
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  2. Jun Kuroda
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  3. Taro Nomura
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Correspondence to Toshio Fukai.

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Fukai, T., Kuroda, J. & Nomura, T. Accurate mass measurement of low molecular weight compounds by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Am Soc Mass Spectrom 11, 458–463 (2000). https://doi.org/10.1016/S1044-0305(00)00109-4

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  • DOI: https://doi.org/10.1016/S1044-0305(00)00109-4

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