Finite Element Analysis of Cardiac Myocyte Debonding and Reorientation During Cyclic Substrate Stretch Experiments
The substrate stretch experiment, which is carried out on several kinds of adherent cells, is usually used to catch the physiological variation and morphological response to cyclic substrate deformation. In this paper, stretch loading was exerted on cardiac myocytes cultured on silica substrates using a custom-made substrate stretch device. The effect of stretch on the alignment orientation of cardiac myocytes was studied through morphocytological statistics. Under cyclic stretch stimulus, the long axes of cardiac myocytes oriented perpendicularly to the stretch direction for continuous stretch acting. However, the mechanism underlying these behaviors is not well understood from such in vitro tests. Finite element (FE) model was developed in the analysis to investigate these behaviors. Xu-Needleman formulation was used to define the interaction behavior for contact surfaces between cell and substrate. The role of cell viscoelasticity nature is studied in adherent cell debonding with the substrate and aligning perpendicular to the stretch direction during long time cyclic stretch stimulation. There were four different strain magnitudes considered in the simulation to find out the cell debonding affected by the cyclic strains. The potential role of cyclic strain frequency in regulating cell debonding and alignment was also studied using FE analysis.
Key wordscardiac myocyte cyclic substrate stretch cell adhesion finite element
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- Standley, P.R., Cammarata, A., Nolan, B.P., Purgason, C.T. and Stanley, M.A., Cyclic stretch induces vascular smooth muscle cell alignment via NO signaling. American Journal of Physiology: Heart and Circulatory Physiology, 2002, 283: 1907–1914.Google Scholar
- Sadoshima, J., Jahn, L., Takahashi, T., Kulik, T.J. and Izumo, S., Molecular characterization of the stretch-induced adaptation of cultured cardiac cells. An in vitro model of load-induced cardiac hypertrophy. Journal of Biological Chemistry, 1992, 267: 10551–10560.Google Scholar
- Spector, D.L. (ed), Goldman, R.D. and Leinwand, L.A., Cells: A Laboratory Manual. America: Cold Spring Harbor Laboratory Press, 1998.Google Scholar
- Fung, Y.C., Foundations of Solid Mechanics. New York: Prentice-Hall Inc., 1965.Google Scholar
- Ferry, J.D., Viscoelastic Properties of Polymers, 3rd edition. New York: Wiley, 1980.Google Scholar
- ABAQUS, ABAQUS User’s Manual Version 6.4. USA: ABAQUS, Inc., 2003.Google Scholar
- Fang, H.R., Tang, T., Zhang, X.M. and Zhuang, Z., Development on the visco-elastic constitutive model of cardiac muscle based on experiment. Acta Mechanica Sinica, 2008, 40(3): 355–363 (in Chinese).Google Scholar
- Lieber, S.C., Aubry, N., Pain, J., Diaz, G., Kim, S.J. and Vatner, S.F., Aging increases stiffness of cardiac myocytes measured by atomic force microscopy nanoindentation. American Journal of Physiology: Heart and Circulatory Physiology, 2004, 287: H645–H651.Google Scholar