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Detection and In Vivo Hormonal Regulation of Rat Ovarian Type I and Type II Interleukin-I Receptor mRNAs: Increased Expression During the Periovulatory Period

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Abstract

Objective

To study the expression, localization, and in vivo hormonal regulation of type I and type II interleukin-1 (IL-1) receptors in the rat ovary.

Methods

Segments of the cDNAs for rat type I and type II IL-1 receptors were cloned and used as probes in RNase protection assays and in situ hybridization. Tissues obtained from immature rats and hormonally treated rat ovaries were examined.

Results

Type I IL-1 receptor (IL-1R(1)) was ubiquitously expressed in rat tissues, including gran-ulosa cells prepared from immature ovaries, whereas type II IL-1 receptor (IL-1R(2)) expression was restricted to macrophages, thymus, and lung. Hypophysectomy and subsequent treatment with FSH and/or diethylstilhestrol did not alter significantly the abundance of IL-1R(1) transcripts in the whole ovary. However, the relative amount of ovarian IL-1R(1) transcripts increased 7.3-fold 6 hours after the administration of hCG to pregnant mare serum gonadotropin-primed immature rats. During this time, IL-1R(1) mRNA was localized primarily in the granulosa cells. The increased expression of IL-1R(l) persisted 24 hours after hCG administration but declined to baseline by 48 hours. Ovarian expression of IL-1R(2) mRNA was observed only before ovulation in amounts that were approximately 70-fold lower than IL-1R(1).

Conclusion

The increased intraovarian expression of IL-1R(1) in granulosa cells during the periovulatory period implies that this cell type has a heightened receptivity to IL-1 and provides further indirect evidence that this cytokine is involved in the ovulatory process.

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Author information

Author notes

  1. Wendy J. Scherzer MD

    Present address: Current address: Department of Ob/Gyn, Temple University, Philadelphia, Pennsylvania, USA

  2. Lechoslaw T. Putowski MD, PhD

    Present address: Current address: 2nd Clinic of Surgical Gynecology, Lublin, Poland

  3. Shahar Kol MD

    Present address: Current address: Department of Obstetrics and Gynecology, Rambam Medical Center, Haifa, Israel

Authors and Affiliations

  1. Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, University of Maryland School of Medicine, 655 W. Baltimore Street, Room 11-011, Baltimore, Maryland, 21201-1559, USA

    Wendy J. Scherzer MD, Kristiina S. L. Ruutiainen-Altman MD, PhD, Lechoslaw T. Putowski MD, PhD, Shahar Kol MD, Eli Y. Adashi MD & Richard M. Rohan PhD

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  1. Wendy J. Scherzer MD
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  2. Kristiina S. L. Ruutiainen-Altman MD, PhD
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  3. Lechoslaw T. Putowski MD, PhD
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  5. Eli Y. Adashi MD
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Additional information

Supported in part by an AFS-Ortho Award (WJS), an NIH Fogarty Fellowship and an award from the Finnish Culture Foundation (KRA), a Fulbright Fellowship (LTP), the Frank C. Bressler Memorial Fund (RMR), and NIH Research Grants HD-30280 and HD-19998 (EYA).

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Scherzer, W.J., Ruutiainen-Altman, K.S.L., Putowski, L.T. et al. Detection and In Vivo Hormonal Regulation of Rat Ovarian Type I and Type II Interleukin-I Receptor mRNAs: Increased Expression During the Periovulatory Period. Reprod. Sci. 3, 131–139 (1996). https://doi.org/10.1016/1071-5576(96)00010-X

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  • DOI: https://doi.org/10.1016/1071-5576(96)00010-X

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