Abstract
Collision-induced dissociation product ion spectra of a series of doubly charged tryptic peptide ions produced by electrospray ionization were obtained by triple-quadrupole tandem mass spectrometry. The sequence information content of the product ion spectra was explored as a function of collision energy and collision-cell gas pressure for parent ions with molecular masses ranging from 300 to 2000 u. The energy range (at a given pressure) in which the degree of fragmentation is acceptable was found to be narrow for parent ions of a given mass, and the optimal collision energy was observed to exhibit a strong linear correlation with parent ion mass. This observed correlation opens the way for on-line software-controled selection of optimal mass spectrometric conditions in the enzymatic digestion-liquid chromatography-tandem mass spectrometric strategy of amino acid sequencing of proteins.
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Haller, I., Mirza, U.A. & Chait, B.T. Collision induced decomposition of peptides. Choice of collision parameters. J Am Soc Mass Spectrom 7, 677–681 (1996). https://doi.org/10.1016/1044-0305(96)85613-3
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DOI: https://doi.org/10.1016/1044-0305(96)85613-3