Abstract
This report describes the fragmentation processes for peptides induced by collisional activation of the 12C isobar of matrix-assisted laser desorption ionization (MALDI)-generated pseudomolecular ions employing an EBE orthogonal acceleration time-of-flight mass spectrometer and using xenon as the collision gas at a laboratory collision energy of 800 eV. These MALDI-collision-induced dissociation (CID) spectra are shown to provide sequence information of comparable quality to those obtained by using high energy CID conditions with liquid secondary ionization mass spectrometry on a four-sector tandem instrument. Peptide sequencing via MALDI-CID is demonstrated on three tryptic peptides obtained from a bacterial protein (P450 isozyme) of unknown sequence. Sensitivity is shown to be at the 1 pmol level for standard peptides.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Burlingame, A. L.; Boyd, R. K.; Gaskell, S. J. Anal. Chem. 1994, 66, 634R.
Burlingame, A. L.; Carr, S. A., eds. Mass Spectrometry in the Biological Sciences; Humana Press: Clifton, NJ, 1996.
Bean, M. F.; Carr, S. A.; Thorne, G. C.; Reilly, M. H.; Gaskell, S. J. Anal. Chem. 1991, 63, 1473.
Biemann, K. Methods Enzymol. 1990, 193, 455–479.
Medzihradszky, K. F.; Burlingame, A. L. Methods: A Companion to Methods in Enzymology 1994, 6, 284.
Beavis, R. C.; Chait, B. T. Proc. Natl. Acad. Sci. USA 1990, 84, 6873.
Henzel, W. J.; Grimley, C.; Bourell, J. H.; Billeci, T. M.; Wong, S. C.; Stults, J. T. Methods: A Companion to Methods in Enzymology 1994, 6, 239–247.
Clauser, K. R.; Hall, S. C.; Smith, D. M.; Webb, J. W.; Andrews, L. E.; Tran, H. M.; Epstein, L. B.; Burlingame, A. L. Proc. Natl. Acad. Sci. USA 1995, 92, 5072.
Strobel, F. H.; Preston, L. M.; Washburn, K. S.; Russell, D. H. Anal. Chem. 1992, 64, 754.
Strobel, F. H.; Russell, D. H. Anal. Chem. 1992, 64, 2879.
Strobel, F. H.; Solouki, T.; White, M. A.; Russell, D. H. J. Am. Soc. Mass Spectrom. 1991, 2, 91.
Strobel, F. H. ; Russell, D. H. Proceedings of the 38th ASMS Conference on Mass Spectrometry and Allied Topics; Washington, DC, 1992; pp 348–349.
Annan, R. S.; Köchling, H. J.; Biller, J. E.; Hill, J. A.; Biemann, K. Proceedings of the 40th ASMS Conference on Mass Spectrometry and Allied Topics; Washington, DC, 1992, pp 348–349.
Köchling, H. J.; Hill, J. A.; Biller, J. E.; Biemann, K. Proceedings of the 41st ASMS Conference on Mass Spectrometry and Allied Topics San Francisco, CA, 1993, pp 435a–435b.
Bateman, R. H.; Green, M. R.; Scott, G. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics; Chicago, IL, 1994, p 1034.
Neumann, G. M.; Sheil, M. M.; Derrick, P. J. Z. Naturforsch. 1984, 39a, 584.
Holmes, J. L. Org. Mass Spectrom. 1985, 20, 169.
Burlingame, A. L. Biological Mass Spectrometry: Present and Future; Matsuo, T.; Caprioli, R. M.; Gross, M. L.; Seyama, Y., eds.; Wiley: New York, p 147.
Hunt, D. F.; Yates, J. R. III Shabanowitz, J.; Winston, S.; Hauer, C. R. Proc. Natl. Acad. Sci. USA 1986, 83, 6233.
Falick, A. M.; Hines, W. M.; Medzihradszky, K. F.; Baldwin, M. A.; Gibson, B. W. J. Am. Soc. Mass Spectrom. 1993, 4, 882.
Johnson, R. S.; Martin, S. A.; Biemann, K. Int. J. Mass Spectrom. Ion Processes 1988, 86, 137.
Hayes, R. N.; Gross, M. L. Methods Enzymol. 1990, 193, 237.
Downard, K. M.; Biemann, K. J. Mass Spectrom. 1995, 30, 25.
Thorne, G. C.; Gaskell, S. J. Rapid Commun. Mass Spectrom. 1989, 3, 217.
Jespersen, S.; Niessen, W. M. A.; Tjaden, U. R.; van der Greef, J.; Litborn, E.; Lindber, U.; Roeraade, J. In Mass Spectrometry in the Biological Sciences; Burlingame, A. L.; Carr, S. A., eds.; Humana Press: New York, 1996, pp 217–226.
Martin, S. A. Proceedings of the 37th ASMS Conference on Mass Spectrometry and Allied Topics; Miami, FL, 1989, pp 858–859.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Medzihradszky, K.F., Adams, G.W., Burlingame, A.L. et al. Peptide sequence determination by matrix-assisted laser desorption ionization employing a tandem double focusing magnetic—Orthogonal acceleration time-of-flight mass spectrometer. J Am Soc Mass Spectrom 7, 1–10 (1996). https://doi.org/10.1016/1044-0305(95)00621-4
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/1044-0305(95)00621-4