Abstract
The noncovalent tetrameric association of the protein streptavidin formed by electrospray ionization (ESI) mass spectrometry has been observed intact and dissociated in the gas phase. An extended mass-to-charge ratio range quadrupole mass spectrometer was employed to examine the effects of harsher conditions in the ESI atmosphere-vacuum interface region on the streptavidin tetramer. Thermally induced dissociation caused the mass spectra to exhibit a series of complementary monomer and trimer ions that correspond to decomposition of the tetrameric species. Similar results were obtained with tandem mass spectrometric experiments on a Fourier transform ion cyclotron resonance mass spectrometer by application of sustained off-resonance irradiation (SORI) on a selected tetrameric charge state. The technique of single-frequency quadrupole excitation was used to accomplish selected-ion accumulation of the 14 + charge state of the tetramer during ion injection. Subsequent low energy SORI combined with broadband quadrupole cooling produced the 7 + monomer and 7 + trimer species, as well as the 6 + monomer and 8 + trimer complementary ions. The observed asymmetric breakup of the tetramer is qualitatively explained by using physical models.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Ganem, B.; Li, Y.-T.; Henion, J. J. Am. Chem. Soc. 1991, 113, 6294.
Ganem, B.; Li, Y.-T.; Henion, J. J. Am. Chem. Soc. 1991, 113, 7818.
Katta, V.; Chait, B. T. J. Am. Chem. Soc. 1991, 113, 8534.
Baca, M.; Kent, S. B. H. J. Am. Chem. Soc. 1992, 114, 3992.
Ganguly, A. K.; Pramanik, B. N.; Tsarbopoulos, A.; Covey, T. R.; Huang, E.; Fuhrman, S. A. J. Am. Chem. Soc. 1992, 114, 6559.
Smith, R. D.; Light-Wahl, K. J.; Winger, B. E.; Loo, J. A. Org. Mass Spectrom. 1992, 27, 811.
Jaquinod, M.; Leize, E.; Potier, N.; Albrecht, A.-M.; Shanzer, A.; Van Dorsselaer, A. Tetrahedron Lett. 1993, 34, 2771.
Ganem, B.; Henion, J. D. Chem. Tracts-Org. Chem. 1993, 6, 1.
Smith, R. D.; Light-Wahl, K. J. Biol. Mass Spectrom. 1993, 22, 493.
Huang, E. C.; Pramanik, B. N.; Tsarbopoulos, A.; Reichert, P.; Ganguly, A. K.; Trotta, P. P.; Nagabhushan, T. L.; Covey, T. R. J. Am. Soc. Mass Spectrom. 1993, 4, 624.
Li, Y.-T.; Hsieh, Y.-L.; Henion, J. D.; Ganem, B. J. Am. Soc. Mass Spectrom. 1993, 4, 631.
Loo, J. A.; Holsworth, D. D.; Root-Bernstein, R. S. Biol. Mass Spectrom. 1994, 23, 6.
Tang, X.; Brewer, C. F.; Saha, S.; Chernushevich, I.; Ens, W.; Standing, K. G. Rapid Commun. Mass Spectrom. 1994, 8, 750.
Light-Wahl, K. J.; Winger, B. E.; Smith, R. D. J. Am. Chem. Soc. 1993, 115, 5869.
Light-Wahl, K. J.; Schwartz, B. L.; Smith, R. D. J. Am. Chem. Soc. 1994, 116, 5271.
Loo, J. A.; Udseth, H. R.; Smith, R. D. Rapid Commun. Mass Spectrom. 1988, 2, 207.
Smith, R. D.; Loo, J. A.; Barinaga, C. J.; Edmonds, C. G.; Udseth, H. R. J. Am. Soc. Mass Spectrom. 1990, 1, 53.
Rockwood, A. L.; Busman, M.; Udseth, H. R.; Smith, R. D. Rapid Commun. Mass Spectrom. 1991, 5, 582.
Argaraña, C. E.; Kuntz, I. D.; Birken, S.; Axel, R.; Cantor, C. R. Nucleic Acids Res. 1986, 14, 1871.
Wilchek, M.; Bayer, E. A. Anal. Biochem. 1988, 171, 1.
Schwartz, B. L.; Light-Wahl, K. J.; Smith, R. D. J. Am. Soc. Mass Spectrom. 1994, 5, 201.
Schwartz, B. L.; Light-Wahl, K. J.; Smith, R. D. Proceedings of the 42nd ASMS Conference on Mass Spectrometry and Allied Topics; Chicago, IL, May 29–June 3, 1994; p 19.
Henry, K. D.; Quinn, J. P.; McLafferty, F. W. J. Am. Chem. Soc. 1991, 133, 5447.
Beu, S. C.; Senko, M. W.; Quinn, J. P.; McLafferty, F. W. J. Am. Soc. Mass Spectrom. 1993, 4, 190.
Bruce, J. E.; Anderson, G. A.; Hofstadler, S. A.; Van Orden, S. L.; Sherman, M. S.; Rockwood, A. L.; Smith, R. D. Rapid Commun. Mass Spectrom. 1993, 7, 914.
Gauthier, J. W.; Trautman, T. R.; Jacobson, D. B. Anal. Chim. Acta 1991, 246, 211.
Guan, S.; Wahl, M. C.; Marshall, A. G. J. Chem. Phys. 1994, 300, 6137.
Huang, Y.; Pasa-Tolic, L.; Guan, S.; Marshall, A. G. Anal. Chem. 1994, 66, 4385.
Bruce, J. E.; Van Orden, S. L.; Anderson, G. A.; Hofstadler, S. A.; Sherman, M. S.; Rockwood, A. L.; Smith, R. D. J. Mass Spectrom. 1995, 30, 124.
Chilkoti, A.; Tan, P. H.; Stayton, P. S. Proc. Natl. Acad. Sci. USA 1995, 92, 1754.
Winger, B. E.; Light-Wahl, K. J.; Ogorzalek Loo, R. R.; Udseth, H. R.; Smith, R. D. J. Am. Soc. Mass Spectrom. 1993, 4, 536.
Chowdhury, S. K.; Katta, V.; Chait, B. T. Rapid Commun. Mass Spectrom. 1990, 4, 81.
Gale, D. C.; Smith, R. D. Rapid Commun. Mass Spectrom. 1993, 7, 1017.
Winger, B. E.; Hofstadler, S. A.; Bruce, J. E.; Udseth, H. R.; Smith, R. D. J. Am. Soc. Mass Spectrom. 1993, 4, 566.
Speir, J. P.; Gorman, G. S.; Pitsenberger, C. C.; Turner, C. A.; Wang, P. P.; Amster, I. J. Anal. Chem. 1993, 53, 1746.
Ryce, S. A.; Wyman, R. R. Can. J. Phys. 1970, 48, 2571.
Rockwood, A. L.; Busman, M.; Smith, R. D. Int. J. Mass Spectrom. Ion Processes 1991, 111, 103.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schwartz, B.L., Bruce, J.E., Anderson, G.A. et al. Dissociation of tetrameric ions of noncovalent streptavidin complexes formed by electrospray ionization. J Am Soc Mass Spectrom 6, 459–465 (1995). https://doi.org/10.1016/1044-0305(95)00191-F
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/1044-0305(95)00191-F