Abstract
The data reported show that heavy-atom substitution on matrices that are used for matrix-assisted laser desorption-ionization mass spectrometry enhance [M + H]+ ion yields of both Leu—enkephalin and vitamin B12. Heavy-atom substitution alters the excited state relaxation dynamics of the matrices as indicated by measured decreases in the measured luminescence (fluorescence and/or phosphorescence) observed for heavy atoms that contain matrices relative to the nonheavy-atom-substituted matrices. The results presented are consistent with the direct involvement of an electronically excited state of the matrix in the formation of analyte [M + H]+ ions.
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Preston Schaffter, L.M., Kinsel, G.R. & Russell, D.H. Effects of heavy-atom substituents on matrices used for matrix-assisted laser desorption—Ionization mass spectrometry. J. Am. Soc. Spectrom. 5, 800–806 (1994). https://doi.org/10.1016/1044-0305(94)87002-0
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DOI: https://doi.org/10.1016/1044-0305(94)87002-0