Abstract
The effects of applied voltages and reaction times on negative ion chemical ionization in the quadrupole ion trap are investigated. Mass-selected ejection of undesired reagent ions and selective mass storage of only negative ions are required for practical negative ion chemical ionization. This is achieved by application of rf and dc voltages to the ring electrode to control the mass-to-charge ratios one polarity) of ions stored, as well as by application of a supplemental rf voltage applied across the endcap electrodes to selectively eject ions of a particular mass-to-charge ratio. Even with careful control of these parameters, negative chemical ionization is not as sensitive as electron ionization and positive chemical ionization because of the lack of thermal electrons in the ion trap. Mass selection of the hydroxide anion as a reagent ion and exclusion of all positive ions provide [M − H]− ions with little or no fragmentation for a wide variety of compounds.
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Paul, W.; Reinhard, H.; van Zahn, V. Z. Z. Physik 1959, 256, 1.
Stafford, Jr., G. C.; Kelley, P. E.; Syka, J. E. P.; Reynolds, W. E.; Todd, J. F. J. Int. J. Mass Spectrom. Ion Processes 1984, 60, 85.
Bower, R. P.; Lawson, G.; Todd, J. F. J. J. Chem. Soc. Chem. Commun. 1972, 1179.
Brodbelt, J. S.; Louris, J. N.; Cooks, R. G. Anal. Chem. 1987, 59, 1278.
McLuckey, S. A.; Glish, G. L.; Kelley, P. E. Anal. Chem. 1987, 59, 1670.
Kelley, P. E.; Stafford, Jr., G. C.; Reynolds, W. E.; Louris, J. N.; Todd, J. F. J, Adv. Mass Spectrom. 1985, 208, 869.
Johnson, J. V,; Yost, R. A.; Kelley, P. E.; Bradford, P. C. Anal. Chem. 1990, 62, 2162.
Webe-Grabau, M.; Kelley, P. E.; Syka, J. E. P.; Bradshaw, S. C.; Brodbelt, J. S. Proceedings of the 35th Annual Conference on Mass Spectrometry and Allied Topics; Denver, CO, 1987; p 1114.
Louis, J. N.; Cooks, R. G.; Syka, J. E. P.; Kelley, P. E.; Stafford, Jr., G. C.; Todd, J. F. J. Anal. Chem. 1987, 59, 1677.
Berberich, D. W.; Yost, R. A. Presented at the 1987 South East Regional Meeting of the American Chemical Society, Orlando, FL, 1987.
Berberich, D. W.; Hail, M. E.; Yost, R. A. Proceedings of the 36th Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, CA, 1988; p 1110.
Weber-Grabau, M.; Kelley, P. E.; Bradshaw, S. C.; Hockman, D. J. Proceedings of the 36th Annual Conference on Mass Spectromety and Allied Topics; San Francisco, 1988; p 1106.
Tucker, D. B.; Hameister, C. H.; Bradshaw, S. C.; Hoekman, D. J.; Weber-Grabau, M. Proceedings of the 36th Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, CA, 1988; p 628.
Glish, G. L.; Van Berkel, G. J.; Asano, K. G.; McLuckey, S. A. Proceedings of the 36th Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, CA, 1988; p 1112.
Brodbelt, J. S.; Cooks, K. G. Anal. Chim. Acta 1988, 206, 239.
Berberich, D. W.; Hail, M. E.; Johnson, J. V.; Yost, R. A. Int. J. Mass Spectrom. Ion. Processes 1989 , 94, 115.
March, R. E.; Hughes, R. Practical Organic Mass Spectrometry; Wiley: New York, 1989.
Strife, R. J.; Keller, P. R. Org. Mass Spectrom. 1989, 24, 201.
Louis, J. N.; Brodbelt-Lustig, J. S.; Cooks, R. G.; Glish, G. L.: Van Berkel, G. J.; McLuckey, S. A. Int. J. Mass Spectrom. Ion Processes 1990, 96, 117.
Strife, R. J.; Simms, J. R.; Lacey, M. P. J. Am. Soc. Mass Spectrom. 1990, 1, 265.
Strife, R. J.; Simms, J. R. J. Am. Soc. Mass Spectrom. 1992, 3, 372.
Donovan, T.; Lieu, C. C.; Brodbelt, J. S. J. Am. Soc. Mass Spectrom. 1992, 3, 39.
Donovan, T.; Brodbelt, J. S. Biol. Mass Spectrom. 1992, 21, 254.
Liou, C. C.; Brodbelt, J. S. J. Am. Chem. Soc. 1992, 114, 6761.
Harrison, A. G. Chemical Ionization Mass Spectromcty, 2nd ed.; CRC Press: Boca Raton, FL, 1992; p 1.
Mather, R. E.; Todd, J. F. J. Int. J. Mass Spectrom. Ion Phys. 1980, 33, 159.
Schermann, J. P.; Major, F. G. Appl. Phys. 1978, 16, 225.
Berberich, D. W.; Yost. R. A. Proceedings of the 37th Annual Conference on Mass Spectrometry and Allied Topics; Miami Beach, 1989; p 1250.
Berberich, D. W.; Orth, R. G. Proceedings of the 38th Annual Confererence on Mass Spectrometry and Allied Topics; Tucson, 1990; p 871.
Eckenrode, B. A.; McLuckey, S. A.; Glish, G. L. Proceedings of the 38th Annual Conference on Mass Spectrometry and Allied Topics; Tucson, 1990; p 869.
Eckenrode, B. A.; Glish, G. L.; McLuckey, S. A. Int. J. Mass Spectrom. Ion Processes 1990, 99, 151.
Dawson, P. H. Quadrupole Mass Spectrometry and Its Applications; Elsevier: Amsterdam, 1976; p 1.
Todd, J. F. J. In Dynamic Mass Spectrometry; Price, D.; Todd, J. F. J., Eds.; Heyden: London, 1981; p 44.
Dillard, J. G. Chem. Rev. 1973, 73, 589.
Dougherty, R. C. Anal. Chem. 1981, 53, 625A.
Pedder, R. E.; Yost, R. A. Proceedings of the 36th Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, 1988, p 632.
Stafford, G. C.; Reeher, J. R.; Smith, R. D.; Story, M. S. In Dynamic Mass Spectrometry; Price, D.; Todd, J. F. J., Eds.; Heyden: London, 1978; Vol. 5, p 55.
Bradenberger, H. In Recent Developments in Mass Spectrometry in Biochemisty and Medicine: Frigerio, A., Ed.; Plenum: New York, 1979; p 85.
Chapman, J. R. Practical Organic Mass Spectromety; Wiley: New York, 1985; p 46.
Jennings, K. R. Anal. Proc. 1980, 18, 320.
Harrison, A. G. Chemical Ionization Mass Spectrometry; CRC Press: Boca Raton, FL, 1983; p 1
Dougherty, R. C.; Daulton, J.; Biros, F. J. Org. Mass Spectrom. 1972, 6, 1171.
Boand, G.; Houriet, R.; Gaumann, T. In Advances In Mass Spectrornetry; Quayle, A., Ed.; Heyden: London, 1980; Vol. 8A, p 238.
Melton, C. E. J. Chem. Phys. 1972, 57, 4218.
Smit, A. L. C.; Field, F. H. J. Am. Chem. Soc. 1977, 99, 6471.
Asano, K. G.; Goeringer, D. E.; McLuckey, S. A.; Hockman, D.; Stiller, S. W. Proceedings of the 41st Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, 1993; p 700a.
Shaffer, B. A,; Karnicky, J.; Buttrill, Jr., S. E. Proceedings of the 41st Annual Conference on Mass Spectrometry and Allied Topics; San Francisco, 1993, p 802a.
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Berberich, D.W., Yost, R.A. Negative chemical ionization in quadrupole ion trap mass spectrometry: Effects of applied voltages and reaction times. J Am Soc Mass Spectrom 5, 757–764 (1994). https://doi.org/10.1016/1044-0305(94)80008-1
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DOI: https://doi.org/10.1016/1044-0305(94)80008-1