Abstract
Capillary zone electrophoresis coupled to continuous flow-fast atom bombardment mass spectrometry is shown to have utility for the detection and characterization of adducts formed by the covalent attachment of four polyaromatic hydrocarbon (PAH) and amino-PAH compounds to deoxyguanosine. Normal scanning provided structural information for a 1.3 ng injection of a model adduct, while 1.3 ng of each of a mixture of adducts was sufficient to determine their molecular weights by monitoring the constant neutral loss of deoxyribose. Exploitation of this loss in the multiple reaction monitoring mode resulted in the detection of low picogram amounts of target adducts in mixtures.
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Randerath, K.; Reddy, M. V.; Gupta, R. C. Proc. Natl. Acad. Sci USA 1981, 78, 6126–6129.
Gupta, R. C.; Reddy, M. V.; Randerath, K. Carcinogenesis 1982, 3 1081–1092.
Harris, C. C.; Yolken, R. H.; Hsu, I.-C. Methods Cancer Res. 1982, 20, 213–243.
Roberts, D. W.; Benson, R. W.; Groopman, J. D.; Flammang, T. J.; Nagle, W. A.; Moss, A. T.; Kadlubar, F. F. Cancer Res. 1988, 48, 6336–6342.
Vahakangas, K.; Trivers, G.; Rowe, M.; Harris, C. C. Env. Health Persp. 1985, 62, 101–104.
Jankowiak, R.; Small, G. J. Anal. Chem. 1989, 61, 1023A-1032A.
Dietrich, A. M.; Guenat, C. R.; Tomer, K. B.; Ball, L. M. Carcinogenesis 1988, 9, 2113–2119.
Rogan, E. C.; Cavalieri, E. L.; Tibbels, S. R.; Cremonesi, P.; Warner, C. D.; Nagel, D. L.; Tomer, K. B.; Cerny, R. L.; Gross, M. L. J. Am. Chem. Soc. 1988, 110, 4023–4029.
Dino, J. J.; Cuenat, C. R.; Tomer, K. B.; Kaufman, D. G. Rapid Comm. Mass Spec. 1987, 1, 69–71.
Tomer, K. B.; Gross, M. L.; Deinzer, M. L. Anal. Chem. 1986, 58, 2527–2534.
Tondeur, Y.; Moschel, R. C.; Dipple, A.; Koepke, S. R. Anal Chem. 1986, 56, 1316–1324.
Annan, R. S.; Giese, R. W.; Vouros, P. Anal. Biochem. 1990, 191, 86–95.
Pavlovich, J. G.; Barofsky, E.; Jiang, L. F.; Barofsky, D. F. Proceedings of the 38th Annual ASMS Conference on Mass Spectrometry and Applied Topics; Nashville, TN, 1991; pp 80–81.
Chiarelli, M. P.; Bryant, M. S.; Lay, J. O. Proceedings of the 38th Annual ASMS Conference on Mass Spectrometry and Allied Topics; Nashville, TN, 1991; pp 967–968.
Pogracz, K.; Kaur, S.; Burlingame, A. L.; Bodell, W. J. Carcinogenesis 1989, 10, 1009–1013.
Fu, P. P.; Miller, D. W.; Von Tungeln, L. S.; Bryant, M. S.; Lay, J. O.; Huang, K.; Jones, L.; Evans, F. E. Carcinogenesis 1991, 12, 609–616.
Mitchum, R. K.; Evans, F. E.; Freeman, J. F.; Roach, D. Int. J. Mass Spectrom. Ion Proc. 1983, 46, 383–386.
Dolnikowski, G. G.; Gross, M. L.; Cavalieri, E. L. J. Am. Soc. Mass Spectrom. 1991, 2, 256–258.
Phillips, D. H. In The Molecular Basis of Cancer; Farmer, P. B.; Walker, J. M., Eds.; Wiley-Interscience: New York, 1985; pp 133–179.
Chacko, M.; Gupta, R. C. Carcinogenesis 1988, 9, 2309–2313.
Ito, Y.; Takeuchi, T.; Ishii, D.; Goto, M. J. Chrom. 1985, 346, 161–166.
Caprioli, R. M.; Fan, T.; Cottrell, J. S. Anal Chem. 1986, 58, 2949–2954.
Continuous-flow Fast Atom Bombardment Mass Spectrometry; Caprioli, R. M., Ed.; John Wiley; New York, 1990.
Reinhoud, N. J.; Niessen, W. M. A.; Tjaden, U. R.; Gramberg, L. G.; Verheij, E. R.; van der Greef, J. Rapid Comm. Mass Spec. 1989, 3, 348–351.
Caprioli, R. M.; Moore, W. T.; Martin, M.; DaGue, B. B.; Wilson, K.; Moring, S. J. Chrom. 1989, 480, 247–257.
Mosley, M. A.; Deterding, L. J.; Tomer, K. B.; Jorgenson, J. W. J. Chrom. 1989, 480, 197–209.
Mosely, M. A.; Deterding, L. J.; Tomer, K. B.; Jorgenson, J, W. Anal Chem. 1991, 63, 109–114.
Wolf, S. M.; Annan, R. S.; Giese, R. G.; Vouros, P. Biol. Mass Spectrom., accepted.
Jackim, E.; Norwood, C. J. High Res. Chrom. 1990, 23, 195–196.
Heflich, R. H.; Djuric, Z.; Zhuo, Z.; Fullerton, N. F.; Casciano, D. A.; Beland, F. A. Environ. Molec. Mutagen. 1988, 11, 167–181.
Casale, R.; McLaughlin, L. W. J. Am. Chem. Soc. 1990, 112, 5264–5271.
Shapiro, R.; Underwood, G. R.; Zawadzka, H.; Broyde, S.; Hingerty, B. E. Biochemistry 1986, 25, 2198–2205.
Kriek, E.; Wesrra, J. G. Carcinogenesis 1980, 1, 459–468.
Declos, K. B.; Miller, D. W.; Lay, J. O.; Casciano, D. A.; Walker, R. P.; Fu, P. P.; Kadlubar, F. F. Carcinogenesis 1987, 8, 1703–1709.
Gupta, R. C. Proc. Natl. Acad. Sci. 1984, 81, 6943–6947.
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Wolf, S.M., Vouros, P., Norwood, C. et al. Identification of deoxynucleoside—polyaromatic hydrocarbon adducts by capillary zone electrophoresis—Continuous Flow-fast atom bombardment mass spectrometry. J Am Soc Mass Spectrom 3, 757–761 (1992). https://doi.org/10.1016/1044-0305(92)87089-H
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DOI: https://doi.org/10.1016/1044-0305(92)87089-H