Mammalian Biology

, Volume 75, Issue 4, pp 348–352 | Cite as

Confirmation of wolf residence in Northern Hungary by field and genetic monitoring

  • Roland Hausknecht
  • Ádám Szabó
  • Gábor Firmánszky
  • Roman Gula
  • Ralph KuehnEmail author
Short Communication


Canis lupus Snow-tracking Noninvasive genetic monitoring mtDNA lineage 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aspi, J., Roininen, E., Ruokonen, M., Kojola, I., Vilá, C., 2006. Genetic diversity, population structure, effective population size and demographic history of the Finnish wolf population. Mol. Ecol. 15, 1561–1576.CrossRefGoogle Scholar
  2. Bandelt, H.J., Forster, P., Roehl, A., 1999. Median-joining networks for inferring intraspecific phylogenies. Mol. Biol. Evol. 16, 37–48.CrossRefGoogle Scholar
  3. Beja-Pereira, A., Oliveira, R., Alves, P.C., Schwartz, M.K., Luikart, G., 2009. Advancing ecological understanding through technological transformations in noninvasive genetics. Mol. Ecol. 9, 1279–1301.CrossRefGoogle Scholar
  4. Broquet, T., Ménard, N., Petit, E., 2007. Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates. Conserv. Genet. 8, 249–260.CrossRefGoogle Scholar
  5. Cercueil, A., Bellemain, E., Manel, S., 2002. PARENTE: a computer program for parentage analysis. J. Hered. 93, 458–459.CrossRefGoogle Scholar
  6. Drummond, A.J., Ashton, B., Cheung, M., Heled, J., Kearse, M., Moir, R., Stones-Havas, S., Thierer, T., Wilson, A., 2007. Geneious v3.0. (available from
  7. Fabbri, E., Miquel, C., Lucchini, V., Santini, A., Caniglia, R., Duchamp, C., Weber, J.M., Lequette, B., Marucco, F., Boitani, L., Fumagalli, L., Taberlet, P., Randi, E., 2007. From the Apennines to the Alps: colonization genetics of the naturally expanding Italian wolf (Canis lupus) population. Mol. Ecol. 16, 1661–1671.CrossRefGoogle Scholar
  8. Farago, S., 1993. Current status of the wolf (Canis lupus L.) in Hungary. In: Promberger, C., Schroder, W. (Eds.), Wolves in Europe — Status and Prospects. Munich Wildlife Society, Munich, pp. 45–49.Google Scholar
  9. Gula, R., 2008a. Wolves return to Poland’s Holy Cross Primeval Forest. International Wolf Magazine, Spring 2008, 17–21.Google Scholar
  10. Gula, R., 2008b. Wolf depredation on domestic animals in the Polish Carpathian Mountains. J. Wildl. Manage. 72, 283–289.CrossRefGoogle Scholar
  11. Gula, R., Hausknecht, R., Kuehn, R., 2009. Evidence of wolf dispersal in anthropogenic habitats of the Polish Carpathian Mountains. Biodivers. Conserv. 18, 2173–2184.CrossRefGoogle Scholar
  12. Hausknecht, R., Gula, R., Pirga, B., Kuehn, R., 2007. Urine — a source for noninvasive genetic monitoring in wildlife. Mol. Ecol. Notes 7, 208–2212.CrossRefGoogle Scholar
  13. Hausknecht, R., Salzer, K., Filli, F., Kuehn, R., 2009. Techniques for noninvasive genetic monitoring of Alpine Ibex Capra ibex. Acta Theriol. 54, 277–287.CrossRefGoogle Scholar
  14. Jedrzejewski, W., Branicki, W., Veit, C., Medugorac, I., Pilot, M., Bunevich, A.N., Jedrzejewska, B., Schmidt, K., Theuerkauf, J., Okarma, H., Gula, R., Szymura, L., Förster, M., 2005. Genetic diversity and relatedness within packs in an intensely hunted population of wolves Canis lupus. Acta Theriol. 50, 3–22.CrossRefGoogle Scholar
  15. Kojola, I., Aspi, J., Hakala, A., Hiekkinen, S., Ilmoni, C., Ronkainen, S., 2006. Dispersal in an expanding wolf population in Finland. J. Mammal. 87, 281–286.CrossRefGoogle Scholar
  16. LIFE 00/NAT/H/7162,2001. ‘Founding the base of long term large carnivore conservation in Hungary’; Department of Wildlife Biology and Game Management, Szent István University, Gödöllő, May.Google Scholar
  17. Lucchini, V., Fabbri, E., Marucco, F., Ricci, S., Boitani, L., Randi, E., 2002. Noninvasive molecular tracking of colonizing wolf (Canis lupus) packs in the western. Italian Alps Mol. Ecol. 11, 857–868.CrossRefGoogle Scholar
  18. Mech, L.D., Boitani, L., 2003. Wolf social ecology. In: Mech, L.D., Boitani, L. (Eds.), Wolves, Ecology and Conservation. The University of Chicago Press, Chicago, USA.CrossRefGoogle Scholar
  19. Pilot, M., Jedrzejewski, W., Branicki, W., Sidorovich, V.E., Jedrzejewska, B., Stachura, K., Funk, S.M., 2006. Ecological factors influence population genetic structure of European grey wolves. Mol. Ecol. 15, 4533–4553.CrossRefGoogle Scholar
  20. Pompanon, F., Bonin, A., Bellemain, E., Taberlet, P., 2005. Genotyping errors: causes, consequences and solutions. Nat. Rev. Genet. 6, 847–856.CrossRefGoogle Scholar
  21. Posada, D., Crandall, K.A., 2001. Intraspecific gene genealogies: trees grafting into networks. Trends Ecol. Evol. 16, 37–45.CrossRefGoogle Scholar
  22. Randi, E., Lucchini, V., Christensen, M.F., Mucci, N., Funk, S., Dolf, G., Loeschcke, V., 2000. Mitochondrial DNA variability in Italian and East European wolves: detecting the consequences of small population size and hybridization. Conserv. Biol. 14, 464–473.CrossRefGoogle Scholar
  23. Salvatori, V., Linnell, J., 2005. Report on the conservation status and threats for wolf (Canis lupus) in Europe. Council of Europe T-PVS/Inf 2005, 16.Google Scholar
  24. Santini, A., Lucchini, V., Fabbri, E., Randi, E., 2007. Ageing and environmental factors affect PCR success in wolf (Canis lupus) excremental DNA samples. Mol. Ecol. Notes 7, 955–961.CrossRefGoogle Scholar
  25. Scandura, M., Capitani, C., Iacolina, L., Apollonio, M., 2006. An empirical approach for reliable microsatellite genotyping of wolf DNA from multiple noninvasive sources. Conserv. Genet. 7, 813–823.CrossRefGoogle Scholar
  26. Tamura, K., Dudley, J., Nei, M., Kumar, S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., online 10.1093/molbev/msm092.Google Scholar
  27. Valière, N., 2002. GIMLET: a computer program for analysing genetic individual identification data. Mol. Ecol. Notes 2, 377–379.Google Scholar
  28. Valière, N., Fumagalli, L., Gielly, L., 2003. Long-distance wolf recolonization of France and Switzerland inferred from noninvasive genetic sampling over a period of 10 years. Anim. Conserv. 6, 83–92.CrossRefGoogle Scholar
  29. Vilà, C., Wayne, R.K., 1999. Hybridization between wolves and dogs. Conserv. Biol. 13, 195–198.CrossRefGoogle Scholar
  30. Wayne, R.K., Vilà, C., 2003. Molecular genetic studies of wolves. In: Mech, L.D., Boitani, L. (Eds.), Wolves, Ecology and Conservation. The University of Chicago Press, Chicago, USA.Google Scholar

Copyright information

© Deutsche Gesellschaft für Säugetierkunde 2009

Authors and Affiliations

  • Roland Hausknecht
    • 1
  • Ádám Szabó
    • 2
  • Gábor Firmánszky
    • 2
  • Roman Gula
    • 3
  • Ralph Kuehn
    • 1
    Email author
  1. 1.Molecular Zoology Unit, Chair of ZoologyTechnische Universitaet MuenchenFreisingGermany
  2. 2.Foundation for Large Carnivores in HungarySzögligetHungary
  3. 3.Museum and Institute of ZoologyPolish Academy of SciencesWarsawPoland

Personalised recommendations