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Enhanced characterization of singly protonated phosphopeptide ions by femtosecond laser-induced ionization/dissociation tandem mass spectrometry (fs-LID-MS/MS)

  • Scott A. Smith
  • Christine L. Kalcic
  • Kyle A. Safran
  • Paul M. Stemmer
  • Marcos Dantus
  • Gavin E. Reida
Article

Abstract

To develop an improved understanding of the regulatory role that post-translational modifications (PTMs) involving phosphorylation play in the maintenance of normal cellular function, tandem mass spectrometry (MS/MS) strategies coupled with ion activation techniques such as collision-induced dissociation (CID) and electron-transfer dissociation (ETD) are typically employed to identify the presence and site-specific locations of the phosphate moieties within a given phosphoprotein of interest. However, the ability of these techniques to obtain sufficient structural information for unambiguous phosphopeptide identification and characterization is highly dependent on the ion activation method employed and the properties of the precursor ion that is subjected to dissociation. Herein, we describe the application of a recently developed alternative ion activation technique for phosphopeptide analysis, termed femtosecond laser-induced ionization/dissociation (fs-LID). In contrast to CID and ETD, fs-LID is shown to be particularly suited to the analysis of singly protonated phosphopeptide ions, yielding a wide range of product ions including a, b, c, x, y, and z sequence ions, as well as ions that are potentially diagnostic of the positions of phosphorylation (e.g., ‘a n+1–98’). Importantly, the lack of phosphate moiety losses or phosphate group ‘scrambling’ provides unambiguous information for sequence identification and phosphorylation site characterization. Therefore, fs-LID-MS/MS can serve as a complementary technique to established methodologies for phosphoproteomic analysis.

Keywords

Electron Capture Dissociation Electron Transfer Dissociation Site Assignment Phosphopeptide Analysis Electron Ionization Dissociation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Supplementary material

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Supplementary material, approximately 1082 KB.

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Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Scott A. Smith
    • 4
  • Christine L. Kalcic
    • 4
  • Kyle A. Safran
    • 4
  • Paul M. Stemmer
    • 1
  • Marcos Dantus
    • 4
    • 2
  • Gavin E. Reida
    • 4
    • 3
  1. 1.Institute of Environmental Health SciencesWayne State UniversityDetroitUSA
  2. 2.Department of PhysicsMichigan State UniversityEast LansingUSA
  3. 3.Department of Biochemistry and Molecular BiologyMichigan State UniversityEast LansingUSA
  4. 4.Department of ChemistryMichigan State UniversityEast LansingUSA

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