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The detection of glycosphingolipids in brain tissue sections by imaging mass spectrometry using gold nanoparticles

  • Naoko Goto-Inoue
  • Takahiro Hayasaka
  • Nobuhiro Zaima
  • Yukiyasu Kashiwagi
  • Mari Yamamoto
  • Masami Nakamoto
  • Mitsutoshi Setou
Application Note

Abstract

Glycosphingolipids (GSLs) are amphiphilic molecules consisting of a hydrophilic carbohydrate chain and a hydrophobic ceramide moiety. They appear to be involved primarily in biological processes such as cell proliferation, differentiation, and signaling. To investigate the mechanism of brain function in more detail, a more highly sensitive method that would reveal the GSL distribution in the brain is required. In this report, we describe a simple and efficient method for mapping the distribution and localization of GSLs present in mouse brain sections using nanoparticle-assisted laser desorption/ionization imaging mass spectrometry (IMS). We have developed and tested gold nanoparticles (AuNPs) as a new matrix to maximize the detection of GSLs. A matrix of AuNPs modified with alkylamine was used to detect various GSLs, such as minor molecular species of sulfatides and gangliosides, in mouse brain sections; these GSLs were hardly detected using 2,5-dihydroxybenzoic acid (DHB), which is the conventional matrix for GSLs. We achieved approximately 20 times more sensitive detection of GSLs using AuNPs compared to a DHB matrix. We believe that our new approach using AuNPs in IMS could lead to a new strategy for analyzing basic biological mechanisms and several diseases through the distribution of minor GSLs.

Keywords

Image Mass Spectrometric MALDI Mass Spectrometry Brain Tissue Section Image Mass Spectrometric Conventional Matrix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Naoko Goto-Inoue
    • 1
  • Takahiro Hayasaka
    • 1
  • Nobuhiro Zaima
    • 1
  • Yukiyasu Kashiwagi
    • 1
  • Mari Yamamoto
    • 2
  • Masami Nakamoto
    • 2
  • Mitsutoshi Setou
    • 1
  1. 1.Department of Molecular Anatomy, Molecular Imaging Frontier Research CenterHamamatsu University School of MedicineShizuokaJapan
  2. 2.Osaka Municipal Technical Research InstituteOsakaJapan

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