Gas-phase conformation-specific photofragmentation of proline-containing peptide ions

  • Tae-Young Kim
  • Stephen J. Valentine
  • David E. Clemmer
  • James P. Reilly
Article

Abstract

Singly-protonated proline-containing peptides with N-terminal arginine are photodissociated with vacuum ultraviolet (VUV) light in an ESI linear ion trap/orthogonal-TOF (LIT/o-TOF). When proline is the nth residue from the N-terminus, unusual bn + 2 and an + 2 ions are observed. Their formation is explained by homolytic cleavage of the Cα− C bond in conjunction with a rearrangement of electrons and an amide hydrogen. The latter is facilitated by a proline-stabilized gas-phase peptide conformation.

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Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Tae-Young Kim
    • 1
  • Stephen J. Valentine
    • 1
  • David E. Clemmer
    • 1
  • James P. Reilly
    • 1
  1. 1.Department of ChemistryIndiana UniversityBloomingtonUSA
  2. 2.Division of Chemistry and Chemical EngineeringCalifornia Institute of TechnologyPasadenaUSA

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