Analysis of various organic and organometallic compounds using nanostructure-assisted laser desorption/ionization time-of-flight mass spectrometry (NALDI-TOFMS)

  • Mark F. Wyatt
  • Shujing Ding
  • Bridget K. Stein
  • A. Gareth Brenton
  • R. Hugh Daniels
Application Note


Nanostructure-assisted laser desorption/ionization time-of-flight mass spectrometry (NALDI-TOFMS) has been developed recently as a matrix-free/surface-assisted alternative to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The NALDI surface of silicon nanowires is already very effective for the analysis of small to medium sized, polar organic molecules in positive ion mode. The current study examined this technology for the analysis of several nonpolar organic, organometallic, and ionic compounds in positive ion mode, as well as a fluorinated compound and various acids in negative ion mode. NALDI data are compared and contrasted with MALDI data for the same compounds, and the higher sensitivity of NALDI is highlighted by the successful characterization of two porphyrins for a sample amount of 10 amol per spot.


Porphyrin Porous Silicon Boronic Acid Silicon Nanowires HFIP 
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Supplementary material

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Supplementary material, approximately 654 KB.


  1. 1.
    Tanaka, K.; Waki, H.; Ido, Y.; Akita, S.; Yoshida, Y.; Yoshida, T.; Matsuo, T. Protein and Polymer Analyses up to m/z 100,000 by Laser Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1988, 2, 151–153.CrossRefGoogle Scholar
  2. 2.
    Kinumi, T.; Saisu, T.; Takayama, M.; Niwa, H. Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using an Inorganic Particle Matrix for Small Molecule Analysis. J. Mass Spectrom. 2000, 35, 417–422.CrossRefGoogle Scholar
  3. 3.
    Sunner, J.; Dratz, E.; Chen, Y. C. Graphite Surface Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry of Peptides and Proteins from Liquid Solutions. Anal. Chem. 1995, 67, 4335–4342.CrossRefGoogle Scholar
  4. 4.
    Wei, J.; Buriak, J. M.; Siuzdak, G. Desorption-Ionization Mass Spectrometry on Porous Silicon. Nature 1999, 399, 243–246.CrossRefGoogle Scholar
  5. 5.
    Finkel, N. H.; Prevo, B. G.; Velev, O. D.; He, L. Ordered Silicon Nanocavity Arrays in Surface-Assisted Desorption/Ionization Mass Spectrometry. Anal. Chem. 2005, 77, 1088–1095.CrossRefGoogle Scholar
  6. 6.
    Peterson, D. S. Matrix-Free Methods for Laser Desorption/Ionization Mass Spectrometry. Mass Spectrom. Rev. 2007, 26, 19–34.CrossRefGoogle Scholar
  7. 7.
    Shenar, N.; Martinez, J.; Enjalbal, C. Laser Desorption/Ionization Mass Spectrometry on Porous Silica and Alumina for Peptide Mass Fingerprinting. J. Am. Soc. Mass Spectrom. 2008, 19, 632–644.CrossRefGoogle Scholar
  8. 8.
    Feuerstein, I.; Najam-ul-Haq, M.; Rainer, M.; Trojer, L.; Bakry, R.; Aprilita, N. H.; Stecher, G.; Huck, C. W.; Bonn, G. K.; Klocker, H.; Bartsch, G.; Guttman, A. Material-Enhanced Laser Desorption/Ionization (MELDI)—a New Protein Profiling Tool Utilizing Specific Carrier Materials for Time of Flight Mass Spectrometric Analysis. J. Am. Soc. Mass Spectrom. 2006, 17, 1203–1208.CrossRefGoogle Scholar
  9. 9.
    Shariatgorji, M.; Amini, N.; Ilag, L. L. Silicon Nitride Nanoparticles for Surface-Assisted Laser Desorption/Ionization of Small Molecules. J. Nanopart. Res. 2009, 11, 1509–1512.CrossRefGoogle Scholar
  10. 10.
    Shmigol, I. V.; Alekseev, S. A.; Lavrynenko, O. Y.; Vasylieva, N. S.; Zaitsev, V. N.; Barbier, D.; Pokrovsky, V. A. Chemically Modified Porous Silicon for Laser Desorption/Ionization Mass Spectrometry of Ionic Dyes. J. Mass Spectrom. 2009, 44, 1234–1240.CrossRefGoogle Scholar
  11. 11.
    Go, E. P.; Apon, J. V.; Luo, G. H.; Saghatelian, A.; Daniels, R. H.; Sahi, V.; Dubrow, R.; Cravatt, B. F.; Vertes, A.; Siuzdak, G. Desorption/Ionization on Silicon Nanowires. Anal. Chem. 2005, 77, 1641–1646.CrossRefGoogle Scholar
  12. 12.
    Luo, G. H.; Chen, Y.; Daniels, H.; Dubrow, R.; Vertes, A. Internal Energy Transfer in Laser Desorption/Ionization from Silicon Nanowires. J. Phys. Chem. B 2006, 110, 13381–13386.CrossRefGoogle Scholar
  13. 13.
    Daniels, R. H.; Dikler, S.; Li, E.; Stacey, C. Break Free of the Matrix: Sensitive and Rapid Analysis of Small Molecules Using Nanostructured Surfaces and LDI-TOF Mass Spectrometry. J. Assoc. Lab. Automation 2008, 13, 314–321.CrossRefGoogle Scholar
  14. 14.
    Kang, M.-J.; Pyun, J.-C.; Lee, J.-C.; Choi, Y.-J.; Park, J.-H.; Park, J.-G.; Lee, J.-G.; Choi, H.-J. Nanowire-Assisted Laser Desorption and Ionization Mass Spectrometry for Quantitative Analysis of Small Molecules. Rapid Commun. Mass Spectrom. 2005, 19, 3166–3170.CrossRefGoogle Scholar
  15. 15.
    Piret, G.; Drobecq, H.; Coffinier, Y.; Melnyk, O.; Boukherroub, R. Matrix-Free Laser Desorption/Ionization Mass Spectrometry on Silicon Nanowire Arrays Prepared by Chemical Etching of Crystalline Silicon. Langmuir 2010, 26, 1354–1361.CrossRefGoogle Scholar
  16. 16.
    Guenin, E.; Lecouvey, M.; Hardouin, J. Could a Nano-Assisted Laser Desorption/Ionization Target Improve the Study of Small Organic Molecules by Laser Desorption/Ionization Time-of-Flight Mass Spectrometry? Rapid Commun. Mass Spectrom. 2009, 23, 1395–1400.CrossRefGoogle Scholar
  17. 17.
    Shenar, N.; Cantel, S.; Martinez, J.; Enjalbal, C. Comparison of Inert Supports in Laser Desorption/Ionization Mass Spectrometry of Peptides: Pencil Lead, Porous Silica Gel, DIOS-chip, and NALDI Target. Rapid Commun. Mass Spectrom. 2009, 23, 2371–2379.CrossRefGoogle Scholar
  18. 18.
    Wyatt, M. F.; Havard, S.; Stein, B. K.; Brenton, A. G. Analysis of Transition-Metal Acetylacetonate Complexes by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 2008, 22, 11–18.CrossRefGoogle Scholar

Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Mark F. Wyatt
    • 1
  • Shujing Ding
    • 1
  • Bridget K. Stein
    • 1
  • A. Gareth Brenton
    • 1
  • R. Hugh Daniels
    • 2
  1. 1.EPSRC National Mass Spectrometry Service Centre (NMSSC), Institute of Mass Spectrometry (IMS), School of MedicineSwansea UniversitySingleton ParkUK
  2. 2.Nanosys Inc.Palo AltoUSA

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