Separation of isomers L-alanine and sarcosine in urine by electrospray ionization and tandem differential mobility analysis-mass spectrometry
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Sarcosine, an isomer of L-alanine, has been proposed as a prostate cancer progression biomarker . Both compounds are detected in urine, where the measured sarcosine/alanine ratio has been found to be higher in prostate biopsy-positive group versus controls. We present here preliminary evidence showing that urine samples spiked with sarcosine/alanine can be partially resolved in 3 min via tandem differential mobility analysis-mass spectrometry (DMA-MS). Based on the calibration curves obtained for two mobility peaks, we finally estimate their concentration ratio in urine.
KeywordsCollision Induce Dissociation Sarcosine Mobility Spectrum Differential Mobility Analyzer Collision Induce Dissociation Spectrum
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