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Cerebral bioimaging of Cu, Fe, Zn, and Mn in the MPTP mouse model of Parkinson’s disease using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)

  • Andreas Matusch
  • Candan Depboylu
  • Christoph Palm
  • Bei Wu
  • Günter U. Höglinger
  • Martin K. -H. Schäfer
  • J. Sabine Becker
Article

Abstract

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been established as a powerful technique for the determination of metal and nonmetal distributions within biological systems with high sensitivity. An imaging LA-ICP-MS technique for Fe, Cu, Zn, and Mn was developed to produce large series of quantitative element maps in native brain sections of mice subchronically intoxicated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) as a model of Parkinson’s disease. Images were calibrated using matrix-matched laboratory standards. A software solution allowing a precise delineation of anatomical structures was implemented. Coronal brain sections were analyzed crossing the striatum and the substantia nigra, respectively. Animals sacrificed 2 h, 7 d, or 28 d after the last MPTP injection and controls were investigated.

We observed significant decreases of Cu concentrations in the periventricular zone and the fascia dentata at 2 h and 7d and a recovery or overcompensation at 28 d, most pronounced in the rostral periventricular zone (+40%). In the cortex Cu decreased slightly to −10%. Fe increased in the interpeduncular nucleus (+40%) but not in the substantia nigra. This pattern is in line with a differential regulation of periventricular and parenchymal Cu, and with the histochemical localization of Fe, and congruent to regions of preferential MPTP binding described in the rodent brain.

The LA-ICP-MS technique yielded valid and statistically robust results in the present study on 39 slices from 19 animals. Our findings underline the value of routine micro-local analytical techniques in the life sciences and affirm a role of Cu availability in Parkinson’s disease.

Keywords

Inductively Couple Plasma Mass Spectrometry Substantia Nigra MPTP Laser Ablation Inductively Couple Plasma Mass Spectrometry MPTP Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Society for Mass Spectrometry 2010

Authors and Affiliations

  • Andreas Matusch
    • 2
  • Candan Depboylu
    • 3
  • Christoph Palm
    • 2
  • Bei Wu
    • 1
    • 4
  • Günter U. Höglinger
    • 3
  • Martin K. -H. Schäfer
    • 5
  • J. Sabine Becker
    • 1
  1. 1.Central Division of Analytical ChemistryForschungszentrum JülichJülichGermany
  2. 2.Institute of Neurosciences and Medicine (INM-1 and −2)Forschungszentrum JülichJülichGermany
  3. 3.Experimental Neurology, Department of NeurologyPhilipps UniversityMarburgGermany
  4. 4.Department of Environmental EngineeringZhejiang UniversityHangzhouChina
  5. 5.Department of Molecular Neuroscience, Institute of Anatomy and Cell BiologyPhilipps UniversityMarburgGermany

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